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J. Biol. Chem., Vol. 281, Issue 20, 14280-14287, May 19, 2006

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Glycans on Secretory Component Participate in Innate Protection against Mucosal Pathogens^*

Clémentine Perrier¹, Norbert Sprenger, and Blaise Corthésy²

From the R & D Laboratory of the Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland and Department of Nutrition, Nestlé Research Center, P.O. Box 44, 1000 Lausanne 26, Switzerland

In mucosal secretions, secretory component (SC) is found either free or bound to polymeric IgA within the secretory IgA complex. SC displays numerous and various glycans, which are potential ligands for bacterial compounds. We first established that human SC (hSC) purified from colostrum (hSCcol) or produced in Chinese hamster ovary cells (hSCrec) exhibits the same lectin reactivity. Both forms bind to Clostridium difficile toxin A and functionally protect polarized Caco-2 cell monolayers from the cytopathic effect of the toxin. The interaction is mediated by glycans present on hSC and involves galactose and sialic acid residues. hSCcol and hSCrec were also shown to bind enteropathogenic Escherichia coli adhesin intimin and to inhibit its infectivity on HEp-2 cells in a glycan-dependent manner as well. SC remained operative in the context of the whole secretory IgA molecule and can therefore enhance its Fab-mediated neutralizing properties. On the contrary, hSC did not interact with three different strains of rotavirus (RF, RRV, and SA11). Accordingly, infection of target MA104 cells with these rotavirus strains was not reduced in the presence of either form of hSC tested. Although not a universal mechanism, these findings identify hSC as a microbial scavenger contributing to the antipathogenic arsenal that protects the body epithelial surfaces.

Received for publication, December 5, 2005 , and in revised form, March 15, 2006.

^* This work was supported by Swiss Science Research Foundation Grants 3200-068038 and 3200-109545. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Supported by a grant from the Nestlé Ph.D. Program.

² To whom correspondence should be addressed: R&D Laboratory of the Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Rue du Bugnon, BH 19-650, 1011 Lausanne, Switzerland. Tel.: 41-21-314-07-83; Fax: 41-21-314-07-71; E-mail: blaise.corthesy{at}chuv.ch.

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