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J. Biol. Chem., Vol. 281, Issue 21, 14981-14990, May 26, 2006

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Plasticity in the Composition of the Light Harvesting Antenna of Higher Plants Preserves Structural Integrity and Biological Function^*

Alexander V. Ruban¹, Svetlana Solovieva, Pamela J. Lee, Cristian Ilioaia, Mark Wentworth, Ulrika Ganeteg, Frank Klimmek, Wah Soon Chow^¶, Jan M. Anderson^¶, Stefan Jansson, and Peter Horton

From the Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom, the Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, S-901 87 Umeå, Sweden, and the ^¶Photobioenergetics Group, Research School of Biological Sciences, Australian National University, Canberra, ACT 2601, Australia

Arabidopsis plants in which the major trimeric light harvesting complex (LHCIIb) is eliminated by antisense expression still exhibit the typical macrostructure of photosystem II in the granal membranes. Here the detailed analysis of the composition and the functional state of the light harvesting antennae of both photosystem I and II of these plants is presented. Two new populations of trimers were found, both functional in energy transfer to the PSII reaction center, a homotrimer of CP26 and a heterotrimer of CP26 and Lhcb3. These trimers possess characteristic features thought to be specific for the native LHCIIb trimers they are replacing: the long wavelength form of lutein and at least one extra chlorophyll b, but they were less stable. A new population of loosely bound LHCI was also found, contributing to an increased antenna size for photosystem I, which may in part compensate for the loss of the phosphorylated LHCIIb that can associate with this photosystem. Thus, the loss of LHCIIb has triggered concerted compensatory responses in the composition of antennae of both photosystems. These responses clearly show the importance of LHCIIb in the structure and assembly of the photosynthetic membrane and illustrate the extreme plasticity at the level of the composition of the light harvesting system.

Received for publication, October 20, 2005 , and in revised form, March 21, 2006.

^* This work was supported by grants from the Biotechnology and Biological Sciences Research Council of the United Kingdom and the INTRO2 European Union FP6 Marie Curie Research Training Network. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 44-114-2222713; Fax: 44-114-2222712; E-mail: a.ruban{at}sheffield.ac.uk.

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