HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 23, 15735-15740, June 9, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/23/15735    most recent M512816200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, H. Articles by Livingston, D. M. Search for Related Content PubMed PubMed Citation Articles by Kim, H. Articles by Livingston, D. M. Social Bookmarking                      What's this?

A High Mobility Group Protein Binds to Long CAG Repeat Tracts and Establishes Their Chromatin Organization in Saccharomyces cerevisiae^*

From the Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455

Long CAG repeat tracts cause human hereditary neurodegenerative diseases and have a propensity to expand during parental passage. Unusual physical properties of CAG repeat tracts are thought to contribute to their instability. We investigated whether their unusual properties alter the organization of CAG repeat tract chromatin. We report that CAG repeat tracts, embedded in yeast chromosomes, have a noncanonical chromatin organization. Digestion of chromatin with the restriction enzyme Fnu4HI reveals hypersensitive sites occurring 125 bp apart in the repeat tract. To determine whether a non-histone protein establishes this pattern, we performed a yeast one-hybrid screen using CAG repeat tracts embedded in front of two reporter genes. The screen identified the high mobility group box protein Hmo1. Chromatin immunoprecipitation of epitope-tagged Hmo1 selectively precipitates CAG repeat tracts DNAs that range from 26 to 126 repeat units. Moreover, deletion of HMO1 drastically alters the Fnu4HI digestion pattern of CAG repeat chromatin. These results show that Hmo1 binds to CAG repeat tracts in vivo and establish the basis of their novel chromatin organization.

Received for publication, November 30, 2005 , and in revised form, April 5, 2006.

^* This work was supported by National Institutes of Health Grant NS41823. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1–3.

¹ To whom correspondence should be addressed: Dept. of Biochemistry, Molecular Biology, and Biophysics, 321 Church St. SE, Minneapolis, MN 55455. Tel.: 612-625-1484: Fax: 612-625-2163: E-mail: livin001{at}umn.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. B. Berger, L. Decourty, G. Badis, U. Nehrbass, A. Jacquier, and O. Gadal Hmo1 Is Required for TOR-Dependent Regulation of Ribosomal Protein Gene Transcription Mol. Cell. Biol., November 15, 2007; 27(22): 8015 - 8026. [Abstract] [Full Text] [PDF]

				K. Kasahara, K. Ohtsuki, S. Ki, K. Aoyama, H. Takahashi, T. Kobayashi, K. Shirahige, and T. Kokubo Assembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae Mol. Cell. Biol., October 1, 2007; 27(19): 6686 - 6705. [Abstract] [Full Text] [PDF]