HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 23, 16117-16127, June 9, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/23/16117    most recent M512757200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hecker, C.-M. Articles by Dikic, I. Search for Related Content PubMed PubMed Citation Articles by Hecker, C.-M. Articles by Dikic, I. Social Bookmarking                      What's this?

Specification of SUMO1- and SUMO2-interacting Motifs^*

Christina-Maria Hecker¹², Matthias Rabiller¹, Kaisa Haglund, Peter Bayer³, and Ivan Dikic⁴

From the Institute for Biochemistry II, Goethe University Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany and the Universität Duisburg-Essen, Fachbereich Biologie und Geografie, Strukturelle und Medizinische Biochemie, Universitätsstrasse, 45117 Essen, Germany

SUMO proteins are ubiquitin-related modifiers implicated in the regulation of gene transcription, cell cycle, DNA repair, and protein localization. The molecular mechanisms by which the sumoylation of target proteins regulates diverse cellular functions remain poorly understood. Here we report isolation and characterization of SUMO1- and SUMO2-binding motifs. Using yeast two-hybrid system, bioinformatics, and NMR spectroscopy we define a common SUMO-interacting motif (SIM) and map its binding surfaces on SUMO1 and SUMO2. This motif forms a -strand that could bind in parallel or antiparallel orientation to the ₂-strand of SUMO due to the environment of the hydrophobic core. A negative charge imposed by a stretch of neighboring acidic amino acids and/or phosphorylated serine residues determines its specificity in binding to distinct SUMO paralogues and can modulate the spatial orientation of SUMO-SIM interactions.

Received for publication, November 29, 2005 , and in revised form, March 7, 2006.

^* This work was supported by grants from the Deutsche Forschungsgemeinschaft (DI 931/1-1) and Boehringer Ingelheim Fonds (to I. D.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1–3.

¹ These authors contributed equally to this work.

² Fellow of the Hessian Ministry for Science and Arts.

³ To whom correspondence may be addressed. E-mail: Peter.Bayer{at}uni-due.de. ⁴ To whom correspondence may be addressed. E-mail: Ivan.Dikic{at}biochem2.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. Cho, Y. Lim, and J. A. Golden SUMO Interaction Motifs in Sizn1 Are Required for Promyelocytic Leukemia Protein Nuclear Body Localization and for Transcriptional Activation J. Biol. Chem., July 17, 2009; 284(29): 19592 - 19600. [Abstract] [Full Text] [PDF]

				Y. Percherancier, D. Germain-Desprez, F. Galisson, X. H. Mascle, L. Dianoux, P. Estephan, M. K. Chelbi-Alix, and M. Aubry Role of SUMO in RNF4-mediated Promyelocytic Leukemia Protein (PML) Degradation: Sumoylation of pml and phospho-switch control of its sumo binding domain dissected in living cells J. Biol. Chem., June 12, 2009; 284(24): 16595 - 16608. [Abstract] [Full Text] [PDF]

				H. A. Blomster, V. Hietakangas, J. Wu, P. Kouvonen, S. Hautaniemi, and L. Sistonen Novel Proteomics Strategy Brings Insight into the Prevalence of SUMO-2 Target Sites Mol. Cell. Proteomics, June 1, 2009; 8(6): 1382 - 1390. [Abstract] [Full Text] [PDF]

				S. Okada, M. Nagabuchi, Y. Takamura, T. Nakagawa, K. Shinmyozu, J.-i. Nakayama, and K. Tanaka Reconstitution of Arabidopsis thaliana SUMO Pathways in E. coli: Functional Evaluation of SUMO Machinery Proteins and Mapping of SUMOylation Sites by Mass Spectrometry Plant Cell Physiol., June 1, 2009; 50(6): 1049 - 1061. [Abstract] [Full Text] [PDF]

				J. Sollier, R. Driscoll, F. Castellucci, M. Foiani, S. P. Jackson, and D. Branzei The Saccharomyces cerevisiae Esc2 and Smc5-6 Proteins Promote Sister Chromatid Junction-mediated Intra-S Repair Mol. Biol. Cell, March 15, 2009; 20(6): 1671 - 1682. [Abstract] [Full Text] [PDF]

				R. Budhiraja, R. Hermkes, S. Muller, J. Schmidt, T. Colby, K. Panigrahi, G. Coupland, and A. Bachmair Substrates Related to Chromatin and to RNA-Dependent Processes Are Modified by Arabidopsis SUMO Isoforms That Differ in a Conserved Residue with Influence on Desumoylation Plant Physiology, March 1, 2009; 149(3): 1529 - 1540. [Abstract] [Full Text] [PDF]

				Y. Tateishi, M. Ariyoshi, R. Igarashi, H. Hara, K. Mizuguchi, A. Seto, A. Nakai, T. Kokubo, H. Tochio, and M. Shirakawa Molecular Basis for SUMOylation-dependent Regulation of DNA Binding Activity of Heat Shock Factor 2 J. Biol. Chem., January 23, 2009; 284(4): 2435 - 2447. [Abstract] [Full Text] [PDF]

				N. Sekiyama, T. Ikegami, T. Yamane, M. Ikeguchi, Y. Uchimura, D. Baba, M. Ariyoshi, H. Tochio, H. Saitoh, and M. Shirakawa Structure of the Small Ubiquitin-like Modifier (SUMO)-interacting Motif of MBD1-containing Chromatin-associated Factor 1 Bound to SUMO-3 J. Biol. Chem., December 19, 2008; 283(51): 35966 - 35975. [Abstract] [Full Text] [PDF]

				N. Crosetto, M. Bienko, R. G. Hibbert, T. Perica, C. Ambrogio, T. Kensche, K. Hofmann, T. K. Sixma, and I. Dikic Human Wrnip1 Is Localized in Replication Factories in a Ubiquitin-binding Zinc Finger-dependent Manner J. Biol. Chem., December 12, 2008; 283(50): 35173 - 35185. [Abstract] [Full Text] [PDF]

				J. Zhu, S. Zhu, C. M. Guzzo, N. A. Ellis, K. S. Sung, C. Y. Choi, and M. J. Matunis Small Ubiquitin-related Modifier (SUMO) Binding Determines Substrate Recognition and Paralog-selective SUMO Modification J. Biol. Chem., October 24, 2008; 283(43): 29405 - 29415. [Abstract] [Full Text] [PDF]

				S. R. Holmstrom, S. Chupreta, A. Y.-L. So, and J. A. Iniguez-Lluhi SUMO-Mediated Inhibition of Glucocorticoid Receptor Synergistic Activity Depends on Stable Assembly at the Promoter But Not on DAXX Mol. Endocrinol., September 1, 2008; 22(9): 2061 - 2075. [Abstract] [Full Text] [PDF]

				M. M. Rytinki and J. J. Palvimo SUMOylation Modulates the Transcription Repressor Function of RIP140 J. Biol. Chem., April 25, 2008; 283(17): 11586 - 11595. [Abstract] [Full Text] [PDF]

				C.-H. Woo, T. Shishido, C. McClain, J. H. Lim, J.-D. Li, J. Yang, C. Yan, and J.-i. Abe Extracellular Signal-Regulated Kinase 5 SUMOylation Antagonizes Shear Stress-Induced Antiinflammatory Response and Endothelial Nitric Oxide Synthase Expression in Endothelial Cells Circ. Res., March 14, 2008; 102(5): 538 - 545. [Abstract] [Full Text] [PDF]

				V. Vethantham, N. Rao, and J. L. Manley Sumoylation Modulates the Assembly and Activity of the Pre-mRNA 3' Processing Complex Mol. Cell. Biol., December 15, 2007; 27(24): 8848 - 8858. [Abstract] [Full Text] [PDF]

				C. V. Esguerra, L. Nelles, L. Vermeire, A. Ibrahimi, A. D. Crawford, R. Derua, E. Janssens, E. Waelkens, P. Carmeliet, D. Collen, et al. Ttrap is an essential modulator of Smad3-dependent Nodal signaling during zebrafish gastrulation and left-right axis determination Development, December 15, 2007; 134(24): 4381 - 4393. [Abstract] [Full Text] [PDF]

				K. Uzunova, K. Gottsche, M. Miteva, S. R. Weisshaar, C. Glanemann, M. Schnellhardt, M. Niessen, H. Scheel, K. Hofmann, E. S. Johnson, et al. Ubiquitin-dependent Proteolytic Control of SUMO Conjugates J. Biol. Chem., November 23, 2007; 282(47): 34167 - 34175. [Abstract] [Full Text] [PDF]

				Y. Xie, O. Kerscher, M. B. Kroetz, H. F. McConchie, P. Sung, and M. Hochstrasser The Yeast Hex3{middle dot}Slx8 Heterodimer Is a Ubiquitin Ligase Stimulated by Substrate Sumoylation J. Biol. Chem., November 23, 2007; 282(47): 34176 - 34184. [Abstract] [Full Text] [PDF]

				S. L. H. Miller, E. L. Scappini, and J. O'Bryan Ubiquitin-interacting Motifs Inhibit Aggregation of PolyQ-expanded Huntingtin J. Biol. Chem., March 30, 2007; 282(13): 10096 - 10103. [Abstract] [Full Text] [PDF]

				M. D. Benson, Q.-J. Li, K. Kieckhafer, D. Dudek, M. R. Whorton, R. K. Sunahara, J. A. Iniguez-Lluhi, and J. R. Martens SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5 PNAS, February 6, 2007; 104(6): 1805 - 1810. [Abstract] [Full Text] [PDF]

				M. H. H. Schmidt and I. Dikic Ubiquitin and NEDD8: Brothers in Arms Sci. Signal., November 21, 2006; 2006(362): pe50 - pe50. [Abstract] [Full Text] [PDF]

				G. D. Raffa, J. Wohlschlegel, J. R. Yates III, and M. N. Boddy SUMO-binding Motifs Mediate the Rad60-dependent Response to Replicative Stress and Self-association J. Biol. Chem., September 22, 2006; 281(38): 27973 - 27981. [Abstract] [Full Text] [PDF]