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J. Biol. Chem., Vol. 281, Issue 23, 16178-16188, June 9, 2006

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Opposing Roles of Zyxin/LPP ACTA Repeats and the LIM Domain Region in Cell-Cell Adhesion^*

From the Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84102

Cadherins mediate cell-cell adhesion by linking cell junctions to actin networks. Although several actin regulatory systems have been implicated in cell-cell adhesion, it remains unclear how such systems drive cadherin-actin network formation and how they are regulated to coincide with initiation of adhesion. Previous work implicated VASP in assembly of cell-cell junctions in keratinocytes and the VASP-binding protein zyxin colocalizes with VASP at cell-cell junctions. Here we examine how domains in zyxin and its relative LPP contribute to cell-cell junction assembly. Using a quantitative assay for cell-cell adhesion, we demonstrate that zyxin and LPP function to increase the rate of early cell-cell junction assembly through the VASP-binding ActA repeat region. We also identify the LIM region of zyxin and LPP to be a regulatory domain that blocks function of these proteins. Deletion of the LIM domains drives adhesion and increases VASP level in detergent insoluble cadherin-actin. Dominant-negative zyxin/LPP mutants reduce the rate of adhesion, lower VASP levels in detergent-insoluble cadherin-actin networks, and allow for the accumulation of capping protein at cell-cell contacts. These data implicate the LIM domains of zyxin and LPP in regulating cell-cell junction assembly through VASP.

Received for publication, November 29, 2005 , and in revised form, April 12, 2006.

^* This work was supported by National Institutes of Health Grant GM050877 (to M. C. B.) and by National Institutes of Health Grant T32CA93247 (to M. D. H. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Physiology and Developmental Biology, Brigham Young University, 574 WIDB, Provo, UT 84602. Tel.: 801-422-4998; Fax: 801-422-0700; E-mail: marchansen{at}byu.edu.

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