HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 24, 16279-16288, June 16, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/24/16279    most recent M600682200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stockdale, C. Articles by Owen-Hughes, T. Search for Related Content PubMed PubMed Citation Articles by Stockdale, C. Articles by Owen-Hughes, T. Social Bookmarking                      What's this?

Analysis of Nucleosome Repositioning by Yeast ISWI and Chd1 Chromatin Remodeling Complexes^*

From the Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom

ISWI proteins form the catalytic core of a subset of ATP-dependent chromatin remodeling activities in eukaryotes from yeast to man. Many of these complexes have been found to reposition nucleosomes but with different directionalities. We find that the yeast Isw1a, Isw2, and Chd1 enzymes preferentially move nucleosomes toward more central locations on short DNA fragments whereas Isw1b does not. Importantly, the inherent positioning properties of the DNA play an important role in determining where nucleosomes are relocated to by all of these enzymes. However, a key difference is that the Isw1a, Isw2, and Chd1 enzymes are unable to move nucleosomes to positions closer than 15 bp from a DNA end, whereas Isw1b can. We also find that there is a correlation between the inability of enzymes to move nucleosomes close to DNA ends and the preferential binding to nucleosomes bearing linker DNA. These observations suggest that the accessibility of linker DNA together with the positioning properties of the underlying DNA play important roles in determining the outcome of remodeling by these enzymes.

Received for publication, January 23, 2006 , and in revised form, April 10, 2006.

^* This work was supported by the Wellcome Trust. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1–3.

¹ To whom correspondence should be addressed. Tel.: 44-1382-385796; Fax: 44-1382-388072; E-mail: t.a.owenhughes{at}dundee.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Biswas, R. Dutta-Biswas, and D. J. Stillman Chd1 and yFACT Act in Opposition in Regulating Transcription Mol. Cell. Biol., September 15, 2007; 27(18): 6279 - 6287. [Abstract] [Full Text] [PDF]

				H. Ferreira, J. Somers, R. Webster, A. Flaus, and T. Owen-Hughes Histone Tails and the H3 {alpha}N Helix Regulate Nucleosome Mobility and Stability Mol. Cell. Biol., June 1, 2007; 27(11): 4037 - 4048. [Abstract] [Full Text] [PDF]

				V. K. Gangaraju and B. Bartholomew Dependency of ISW1a Chromatin Remodeling on Extranucleosomal DNA Mol. Cell. Biol., April 15, 2007; 27(8): 3217 - 3225. [Abstract] [Full Text] [PDF]