HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 25, 17164-17172, June 23, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/25/17164    most recent M600927200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Poëa-Guyon, S. Articles by Morel, N. Search for Related Content PubMed PubMed Citation Articles by Poëa-Guyon, S. Articles by Morel, N. Social Bookmarking                      What's this?

Alternative Splicing Controls Neuronal Expression of v-ATPase Subunit a1 and Sorting to Nerve Terminals^*

From the Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS UPR9040, 91198 Gif sur Yvette, France

Vacuolar proton ATPase accumulates protons inside various intracellular organelles such as synaptic vesicles; its membrane domain V0 could also be involved in membrane fusion. These different functions could require vacuolar proton ATPases possessing different V0 subunit a isoforms. In vertebrates, four genes encode isoforms a1–a4, and a1 variants are also generated by alternative splicing. We identified a novel a1 splice variant a1-IV and showed that the two a1 variants containing exon C are specifically expressed in neurons. Single neurons coexpress a2, a1-I, and a1-IV, and these subunit a isoforms are targeted to different membrane compartments. Recombinant a2 was accumulated in the trans-Golgi network, and a1-I was concentrated in axonal varicosities, whereas a1-IV was sorted to both distal dendrites and axons. Our results indicate that alternative splicing of exon N controls differential sorting of a1 variants to nerve terminals or distal dendrites, whereas exon C regulates their neuronal expression.

Received for publication, January 31, 2006 , and in revised form, March 9, 2006.

^* This work was supported in part by Ministère délégué à la Recherche et aux Nouvelles Technologies Grant 04 2 425. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 33-1-69823647; Fax: 33-1-69824141; E-mail: nicolas.morel{at}nbcm.cnrs-gif.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. W. Moreau, M. Amar, N. Le Roux, N. Morel, and P. Fossier Serotoninergic Fine-Tuning of the Excitation-Inhibition Balance in Rat Visual Cortical Networks Cereb Cortex, July 8, 2009; (2009) bhp114v3. [Abstract] [Full Text] [PDF]

				P. Kreis, E. Thevenot, V. Rousseau, B. Boda, D. Muller, and J.-V. Barnier The p21-activated Kinase 3 Implicated in Mental Retardation Regulates Spine Morphogenesis through a Cdc42-dependent Pathway J. Biol. Chem., July 20, 2007; 282(29): 21497 - 21506. [Abstract] [Full Text] [PDF]