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J. Biol. Chem., Vol. 281, Issue 25, 17286-17303, June 23, 2006

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Laminin 3 Forms a Complex with 3 and 3 Chains That Serves as the Ligand for 61-Integrin at the Apical Ectoplasmic Specialization in Adult Rat Testes^*

From the Center for Biomedical Research, Population Council, New York, New York 10021

Apical ectoplasmic specialization (ES) is a testis-specific hybrid cell/cell actin-based adherens junction and cell/matrix focal contact anchoring junction type restricted to the interface between Sertoli cells and developing spermatids. Recent studies have shown that laminin 3, restricted to elongating spermatids, is a putative binding partner of 6 1-integrin localized in Sertoli cells at the apical ES. However, the identity of the and chains, which constitute a functional laminin ligand with the 3 chain at the apical ES, is not known. Using reverse transcription-PCR and immunoblotting to survey all laminin chains in cells of the seminiferous epithelium, it was noted that 2, 3, 1, 2, 3, and 3 chains were found in germ cells, whereas 1, 2, 4, 5, 1, 2, 1, 2, and 3 chains were found in Sertoli cells, implying that 3 and 3 are the plausible laminin chains restricted to germ cells that may be the bona fide partners of 3. To verify this postulate, recombinant proteins based on domain G of 3 and domain I of 3 and 3 chains were produced and used to obtain the corresponding specific polyclonal antibodies. Additional studies have demonstrated that the laminin 3, 3, and 3 chains indeed are restricted to germ cells at the apical ES, co-localizing with each other and with 1-integrin. Furthermore, co-immunoprecipitation studies have confirmed the interactions among laminin 3, 3, and 3, as well as 1-integrin. When the functional laminin ligand at the apical ES was disrupted via blocking antibodies, such as using anti-laminin 3 or 3 IgG, this treatment perturbed adhesion between Sertoli and germ cells (mostly spermatids), leading to germ cell loss from the epithelium. More important, a transient disruption of the blood-testis barrier was also detected.

Received for publication, December 12, 2005 , and in revised form, April 10, 2006.

^* This work was supported by Grants U01 HD045908 and U54 HD029990 (Project 3) (to C. Y. C.) from NICHD, National Institutes of Health, and Grant CICCR CIG01-72 from the CONRAD (Contraceptive Research and Development) Program. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Tables S1-S3 and Fig. S1.

¹ Recipient of a University of Hong Kong postgraduate scholarship research award.

² To whom correspondence should be addressed: Population Council, 1230 York Ave., New York, NY 10021. Fax: 212-327-8733. E-mail: Y-Cheng{at}popcbr.rockefeller.edu.

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