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J. Biol. Chem., Vol. 281, Issue 26, 17999-18007, June 30, 2006

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Protective Effects of Catalase Overexpression on UVB-induced Apoptosis in Normal Human Keratinocytes^*

Hamid Reza Rezvani, Frédéric Mazurier, Muriel Cario-André, Catherine Pain, Cécile Ged, Alain Taïeb, and Hubert de Verneuil¹

From the INSERM E0217, University Victor Segalen Bordeaux 2, Bordeaux F-33000, France and the Centre Hospitalier Universitaire de Bordeaux, Department of Dermatology, Hôpital St André, Bordeaux F-33000, France

UV-induced apoptosis in keratinocytes is a highly complex process in which various molecular pathways are involved. These include the extrinsic pathway via triggering of death receptors and the intrinsic pathway via DNA damage and reactive oxygen species (ROS) formation. In this study we investigated the effect of catalase and CuZn-superoxide dismutase (SOD) overexpression on apoptosis induced by UVB exposure at room temperature or 4 °C on normal human keratinocytes. Irradiation at low temperature reduced UV-induced apoptosis by 40% in normal keratinocytes independently of any change in p53 and with a decrease in caspase-8 activation. Catalase overexpression decreased apoptosis by 40% with a reduction of caspase-9 activation accompanied by a decrease in p53. Keeping cells at low temperature and catalase overexpression had additive effects. CuZn-SOD overexpression had no significant effect on UVB-induced apoptosis. UVB induced an increase in ROS levels at two distinct stages: immediately following irradiation and around 3 h after irradiation. Catalase overexpression inhibited only the late increase in ROS levels. We conclude that catalase overexpression has a protective role against UVB irradiation by preventing DNA damage mediated by the late ROS increase.

Received for publication, January 18, 2006 , and in revised form, March 23, 2006.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 33-5-57-57-13-73; Fax: 33-5-56-98-33-48; E-mail: verneuil{at}u-bordeaux2.fr.

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				H. R. Rezvani, S. Dedieu, S. North, F. Belloc, R. Rossignol, T. Letellier, H. de Verneuil, A. Taieb, and F. Mazurier Hypoxia-inducible Factor-1{alpha}, a Key Factor in the Keratinocyte Response to UVB Exposure J. Biol. Chem., June 1, 2007; 282(22): 16413 - 16422. [Abstract] [Full Text] [PDF]