HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 28, 18942-18952, July 14, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/28/18942    most recent M510870200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mitsunaga, C. Articles by Sugahara, K. Search for Related Content PubMed PubMed Citation Articles by Mitsunaga, C. Articles by Sugahara, K. Social Bookmarking                      What's this?

Chondroitin Sulfate/Dermatan Sulfate Hybrid Chains in the Development of Cerebellum

SPATIOTEMPORAL REGULATION OF THE EXPRESSION OF CRITICAL DISULFATED DISACCHARIDES BY SPECIFIC SULFOTRANSFERASES^*

From the Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558, Japan

Chondroitin sulfate/dermatan sulfate (CS/DS) chains regulate the development of the central nervous system in vertebrates. Previously, we demonstrated that CS/DS hybrid chains from embryonic pig brain exhibit neuritogenic and growth factor binding activities, which depended on their IdoUA content defining the DS-like structure. To elucidate the distribution of such functional sugar chains during the development of the brain, in situ hybridization was performed to examine expression of three CS/DS GalNAc 4-O-sulfotransferases, D4ST-1, C4ST-1, and C4ST-2, and a single uronyl 2-O-sulfotransferase (UST) involved in the biosynthesis of DS in addition to CS intermediates. C4ST-1 and C4ST-2 were ubiquitously expressed in the postnatal mouse brain, whereas the expression of D4ST-1 and UST was restricted in the developing cerebellum and culminated at postnatal day 14 as shown by reverse transcriptase-PCR analysis. In situ analysis of the disaccharides of CS/DS in brain sections revealed that the concentration of CS/DS increases 2-fold during development (postnatal day 7 to 7 weeks). The proportions of DS-specific, principal disaccharides, IdoUA-Gal-NAc(4-O-sulfate) (iA) and IdoUA(2-O-sulfate)-GalNAc(4-O-sulfate) (iB), produced by the sequential actions of D4ST-1 and UST, were higher in the CS/DS chains from cerebellum than those from whole brain sections. A dramatic increase (10-fold) in the proportion of iB during development was noteworthy. In contrast, GlcUA/IdoUA(2-O-sulfate)-GalNAc(6-O-sulfate) (D/iD) and GlcUA/IdoUA-GalNAc(4, 6-O-disulfate) (E/iE) decreased to 50 and 30%, respectively, in the developing cerebellum. These results suggest that the IdoUA-containing iA and iB units along with D/iD and E/iE units in the CS/DS hybrid play important roles in the formation of the cerebellar neural network during postnatal brain development.

Received for publication, October 5, 2005 , and in revised form, March 20, 2006.

^* This work was supported in part by the Science Research Promotion Fund from the Japan Private School Promotion Foundation, and Grant-in-aid for Encouragement of Young Scientists 16790068 (to T. M.), Exploratory Research 15659021 (to K. S.), The Human Frontier Science Program, and Scientific Research-B 16390026 (to K. S.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Both authors contributed equally to this work.

² Supported by the Core Research for Evolutional Science and Technology, Japan Science and Technology Agency. To whom correspondence should be addressed: Faculty of Advanced Life Science, Hokkaido University, Frontier Research Center for Post-Genomic Science and Technology, Nishi 11-choume, Kita 21-jo, Kita-ku, Sapporo 001-0021, Japan. Tel.: 81-11-706-9054; Fax: 81-11-706-9056; E-mail: k-sugar{at}sci.hokudai.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Yamada, M. Onishi, R. Fujinawa, Y. Tadokoro, K. Okabayashi, M. Asashima, and K. Sugahara Structural and functional changes of sulfated glycosaminoglycans in Xenopus laevis during embryogenesis Glycobiology, May 1, 2009; 19(5): 488 - 498. [Abstract] [Full Text] [PDF]

				M. Ishii and N. Maeda Oversulfated Chondroitin Sulfate Plays Critical Roles in the Neuronal Migration in the Cerebral Cortex J. Biol. Chem., November 21, 2008; 283(47): 32610 - 32620. [Abstract] [Full Text] [PDF]

				H. Wang, Y. Katagiri, T. E. McCann, E. Unsworth, P. Goldsmith, Z.-X. Yu, F. Tan, L. Santiago, E. M. Mills, Y. Wang, et al. Chondroitin-4-sulfation negatively regulates axonal guidance and growth J. Cell Sci., September 15, 2008; 121(18): 3083 - 3091. [Abstract] [Full Text] [PDF]

				M. Ishii and N. Maeda Spatiotemporal expression of chondroitin sulfate sulfotransferases in the postnatal developing mouse cerebellum Glycobiology, August 1, 2008; 18(8): 602 - 614. [Abstract] [Full Text] [PDF]