HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 30, 20993-21003, July 28, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/30/20993    most recent M604208200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schweppe, R. E. Articles by Ahn, N. G. Search for Related Content PubMed PubMed Citation Articles by Schweppe, R. E. Articles by Ahn, N. G. Social Bookmarking                      What's this?

Global Gene Expression Analysis of ERK5 and ERK1/2 Signaling Reveals a Role for HIF-1 in ERK5-mediated Responses^*

Rebecca E. Schweppe¹, Tom Hiu Cheung, and Natalie G. Ahn²

From the Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309

ERK5 is a recently characterized MAPK, which is most similar to the well studied ERK1/2 subfamily but uses distinct mechanisms to elicit responses. To understand the specificity of signaling through ERK5 versus ERK1/2, we examined global gene expression changes in response to each pathway. Microarray measurements in retinal pigment epithelial cells revealed 36 genes regulated by ERK5, all which were novel targets for this pathway. 39 genes were regulated by ERK1/2, which included 11 known genes. Of these genes, 19 were regulated by both pathways. Inspection of the 17 genes uniquely regulated by ERK5 revealed that 14 genes (82%) were previously associated with hypoxia via regulation by HIF-1. In contrast, 16 genes (84%) regulated by either ERK5 or ERK1/2 were implicated in hypoxia, most through mechanisms independent of HIF-1. Of the 20 genes regulated by ERK1/2, only 9 were implicated in hypoxia and were not well characterized hypoxia targets. Thus, unlike ERK5, a mechanistic link between ERK1/2 and HIF-1/HRE could not be established on the basis of gene regulation. Activation of both pathways enhanced transcription from a hypoxia-response element and increased HIF-1 protein expression. In contrast, ERK5 but not ERK1/2 elevated transcription through GAL4-HIF-1. Most interestingly, ERK5 is not significantly activated by hypoxia in retinal pigment epithelial cells, indicating that ERK5 regulation of these genes is relevant in normoxia rather than hypoxia. Thus, ERK5 and ERK1/2 differ in their mechanisms of gene regulation, and indicate that ERK5 may control hypoxia-responsive genes by a mechanism independent of HIF-1 expression control.

Received for publication, May 2, 2006

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Tables S1 and S2.

¹ Howard Hughes Medical Institute Fellow of the Life Sciences Research Foundation.

² To whom correspondence should be addressed: Dept. of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309-0215. Tel.: 303-492-4799; Fax: 303-492-2439; E-mail: Natalie.Ahn{at}Colorado.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. L. Deshmane, R. Mukerjee, S. Fan, L. Del Valle, C. Michiels, T. Sweet, I. Rom, K. Khalili, J. Rappaport, S. Amini, et al. Activation of the Oxidative Stress Pathway by HIV-1 Vpr Leads to Induction of Hypoxia-inducible Factor 1{alpha} Expression J. Biol. Chem., April 24, 2009; 284(17): 11364 - 11373. [Abstract] [Full Text] [PDF]

				X.-M. Yang, Y.-S. Wang, J. Zhang, Y. Li, J.-F. Xu, J. Zhu, W. Zhao, D.-K. Chu, and P. Wiedemann Role of PI3K/Akt and MEK/ERK in Mediating Hypoxia-Induced Expression of HIF-1{alpha} and VEGF in Laser-Induced Rat Choroidal Neovascularization Invest. Ophthalmol. Vis. Sci., April 1, 2009; 50(4): 1873 - 1879. [Abstract] [Full Text] [PDF]

				H. Alam, J. Weck, E. Maizels, Y. Park, E. J. Lee, M. Ashcroft, and M. Hunzicker-Dunn Role of the Phosphatidylinositol-3-Kinase and Extracellular Regulated Kinase Pathways in the Induction of Hypoxia-Inducible Factor (HIF)-1 Activity and the HIF-1 Target Vascular Endothelial Growth Factor in Ovarian Granulosa Cells in Response to Follicle-Stimulating Hormone Endocrinology, February 1, 2009; 150(2): 915 - 928. [Abstract] [Full Text] [PDF]

				C. V. Recktenwald, R. Kellner, R. Lichtenfels, and B. Seliger Altered Detoxification Status and Increased Resistance to Oxidative Stress by K-Ras Transformation Cancer Res., December 15, 2008; 68(24): 10086 - 10093. [Abstract] [Full Text] [PDF]