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J. Biol. Chem., Vol. 281, Issue 31, 22248-22260, August 4, 2006

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Fission Yeast Mcm10p Contains Primase Activity^*

From the Program of Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

Although Mcm10p is a conserved essential component in eukaryotes required for both the initiation and elongation of DNA chains, its biochemical properties are unknown. Here, we report that the Schizosaccharomyces pombe fission yeast Mcm10 protein contains primase activity. Primases are enzymes that synthesize RNA primers on single-stranded DNA templates that are extended by DNA polymerases. In keeping with this property, Mcm10p supported oligoribonucleotide synthesis of short RNA primers (preferentially initiating synthesis on a dT template) that were extended with dATP by Escherichia coli DNA polymerase I. The C terminus of Mcm10p synthesized RNA, but less efficiently than the full-length protein at low rNTP levels. Mcm10p homologs contain a C-terminal motif found in proteins that polymerize nucleotides. A point mutant within this motif of S. pombe Mcm10p was defective in primer synthesis in vitro, and this mutant failed to support growth in vivo, suggesting that the primase activity of Mcm10p may be essential for cell viability.

Received for publication, December 6, 2005 , and in revised form, May 4, 2006.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1 and 2 and Table 1.

¹ To whom correspondence may be addressed. Tel.: 212-639-5895; Fax: 212-717-3627; E-mail: fienk{at}mskcc.org.

² To whom correspondence may be addressed: Program of Molecular Biology, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., P. O. Box 97, New York, NY 10021. Tel.: 212-639-5896; Fax: 212-717-3627; E-mail: j-hurwitz{at}ski.mskcc.org.

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