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J. Biol. Chem., Vol. 281, Issue 31, 22332-22341, August 4, 2006

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Association of Catsper1 or -2 with Ca_v3.3 Leads to Suppression of T-type Calcium Channel Activity^*

From the Neuroscience Research and Advanced Technology, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064

Sperm-specific CatSper1 and CatSper2 proteins are critical to sperm-hyperactivated motility and male fertility. Although architecturally resembling voltage-gated ion channels, neither CatSper1 nor CatSper2 alone forms functional ion channels in heterologous expression systems, which may be related to the absence of yet unidentified accessory subunits. Here we isolated CatSper1- and CatSper2-associated protein(s) from human sperm and analyzed their identities by a multidimensional protein identification technology approach. We identified the T-type voltage-gated calcium channel Ca_v3.3 as binding to both CatSper1 and CatSper2. The specificity of their interactions was verified by co-immunoprecipitation in transfected mammalian cells. Electrophysiological studies revealed that the co-expression of CatSper1 or CatSper2 specifically inhibited the amplitude of Ca_v3.3-evoked T-type calcium current without altering other biophysical properties of Ca_v3.3. Immunostaining studies revealed co-localization of CatSper1 and Ca_v3.3 on the principal piece of human sperm tail. Furthermore, fluorescence resonance energy transfer analysis revealed close proximity and physical association of these two proteins on the sperm tail. These studies demonstrate that CatSper1 and CatSper2 can associate with and modulate the function of the Ca_v3.3 channel, which might be important in the regulation of sperm function.

Received for publication, October 17, 2005 , and in revised form, May 1, 2006.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Neuroscience Research, Dept. R4PM, Bldg. AP9-1125, Abbott Laboratories, 100 Abbott Park Rd., Abbott Park, IL 60064-6118. Tel.: 847-937-8271; Fax: 847-937-9195; E-mail: di.zhang{at}abbott.com.

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				J. Jin, N. Jin, H. Zheng, S. Ro, D. Tafolla, K. M. Sanders, and W. Yan Catsper3 and Catsper4 Are Essential for Sperm Hyperactivated Motility and Male Fertility in the Mouse Biol Reprod, July 1, 2007; 77(1): 37 - 44. [Abstract] [Full Text] [PDF]

				H.-G. Li, X.-F. Ding, A.-H. Liao, X.-B. Kong, and C.-L. Xiong Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility Mol. Hum. Reprod., May 1, 2007; 13(5): 299 - 306. [Abstract] [Full Text] [PDF]