HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 32, 22695-22706, August 11, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/32/22695    most recent M511640200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Villagra, A. Articles by Montecino, M. Search for Related Content PubMed PubMed Citation Articles by Villagra, A. Articles by Montecino, M. Social Bookmarking                      What's this?

Chromatin Remodeling and Transcriptional Activity of the Bone-specific Osteocalcin Gene Require CCAAT/Enhancer-binding Protein -dependent Recruitment of SWI/SNF Activity^*

Alejandro Villagra¹, Fernando Cruzat, Loreto Carvallo, Roberto Paredes, Juan Olate, Andre J. van Wijnen, Gary S. Stein, Jane B. Lian, Janet L. Stein, Anthony N. Imbalzano, and Martin Montecino²

From the Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160-C, 4079100 Concepcion, Chile and the Department of Cell Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01655

Tissue-specific activation of the osteocalcin (OC) gene is associated with changes in chromatin structure at the promoter region. Two nuclease-hypersensitive sites span the key regulatory elements that control basal tissue-specific and vitamin D₃-enhanced OC gene transcription. To gain understanding of the molecular mechanisms involved in chromatin remodeling of the OC gene, we have examined the requirement for SWI/SNF activity. We inducibly expressed an ATPase-defective BRG1 catalytic subunit that forms inactive SWI/SNF complexes that bind to the OC promoter. This interaction results in inhibition of both basal and vitamin D₃-enhanced OC gene transcription and a marked decrease in nuclease hypersensitivity. We find that SWI/SNF is recruited to the OC promoter via the transcription factor CCAAT/enhancer-binding protein , which together with Runx2 forms a stable complex to facilitate RNA polymerase II binding and activation of OC gene transcription. Together, our results indicate that the SWI/SNF complex is a key regulator of the chromatin-remodeling events that promote tissue-specific transcription in osteoblasts.

Received for publication, October 27, 2005 , and in revised form, June 7, 2006.

^* This work has been supported by Fondo Nacional de Investigacion en Ciencia y Tecnologia (FONDECYT) Grant 1030749 and CONICYT-PBCT Grant ACT44 (to M. M.), FIRCA-National Institutes of Health (NIH) Grant 5RO3TW00990 (to G. S. S. and M. M.), and NIH Grants PO1AR48818 (to G. S. S.) and DE12528 (to J. B. L.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1S–4S.

¹ Supported by doctoral fellowships from CONICYT and MECESUP UCH9903.

² To whom correspondence should be addressed: Dept. de Bioquimica y Biologia Molecular, Facultad de Ciencias Biologicas, Universidad de Concepcion, Casilla 160-C, 4079100 Concepcion, Chile. Tel.: 56-41-203815; Fax: 56-41-239687; E-mail: mmonteci{at}udec.cl.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Sun, Q. Chen, S. Yang, Q. Pan, J. Ma, Y. Wan, C.-H. Chang, and A. Hong Remodeling of chromatin structure within the promoter is important for bmp-2-induced fgfr3 expression Nucleic Acids Res., July 1, 2009; 37(12): 3897 - 3911. [Abstract] [Full Text] [PDF]

				N. A. Neuman, S. Ma, G. R. Schnitzler, Y. Zhu, G. Lagna, and A. Hata The Four-and-a-half LIM Domain Protein 2 Regulates Vascular Smooth Muscle Phenotype and Vascular Tone J. Biol. Chem., May 8, 2009; 284(19): 13202 - 13212. [Abstract] [Full Text] [PDF]

				Y. Zhong, H. J. Armbrecht, and S. Christakos Calcitonin, a Regulator of the 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase Gene J. Biol. Chem., April 24, 2009; 284(17): 11059 - 11069. [Abstract] [Full Text] [PDF]

				S. Flowers, N. G. Nagl Jr., G. R. Beck Jr., and E. Moran Antagonistic Roles for BRM and BRG1 SWI/SNF Complexes in Differentiation J. Biol. Chem., April 10, 2009; 284(15): 10067 - 10075. [Abstract] [Full Text] [PDF]

				P. Dhawan, R. Weider, and S. Christakos CCAAT Enhancer-binding Protein {alpha} Is a Molecular Target of 1,25-Dihydroxyvitamin D3 in MCF-7 Breast Cancer Cells J. Biol. Chem., January 30, 2009; 284(5): 3086 - 3095. [Abstract] [Full Text] [PDF]

				R. Bouillon, G. Carmeliet, L. Verlinden, E. van Etten, A. Verstuyf, H. F. Luderer, L. Lieben, C. Mathieu, and M. Demay Vitamin D and Human Health: Lessons from Vitamin D Receptor Null Mice Endocr. Rev., October 1, 2008; 29(6): 726 - 776. [Abstract] [Full Text] [PDF]

				A. Ulsamer, Ma. J. Ortuno, S. Ruiz, A. R. G. Susperregui, N. Osses, J. L. Rosa, and F. Ventura BMP-2 Induces Osterix Expression through Up-regulation of Dlx5 and Its Phosphorylation by p38 J. Biol. Chem., February 15, 2008; 283(7): 3816 - 3826. [Abstract] [Full Text] [PDF]

				A. Villagra, N. Ulloa, X. Zhang, Z. Yuan, E. Sotomayor, and E. Seto Histone Deacetylase 3 Down-regulates Cholesterol Synthesis through Repression of Lanosterol Synthase Gene Expression J. Biol. Chem., December 7, 2007; 282(49): 35457 - 35470. [Abstract] [Full Text] [PDF]

				M. Zhang, H. Fang, J. Zhou, and B. P. Herring A Novel Role of Brg1 in the Regulation of SRF/MRTFA-dependent Smooth Muscle-specific Gene Expression J. Biol. Chem., August 31, 2007; 282(35): 25708 - 25716. [Abstract] [Full Text] [PDF]

				J. Gutierrez, R. Paredes, F. Cruzat, D. A. Hill, A. J. van Wijnen, J. B. Lian, G. S. Stein, J. L. Stein, A. N. Imbalzano, and M. Montecino Chromatin Remodeling by SWI/SNF Results in Nucleosome Mobilization to Preferential Positions in the Rat Osteocalcin Gene Promoter J. Biol. Chem., March 30, 2007; 282(13): 9445 - 9457. [Abstract] [Full Text] [PDF]