| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 281, Issue 32, 22992-23002, August 11, 2006
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1
From the
Cystic Fibrosis/Pulmonary Research and Treatment Center, and the Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27599
Extracellular ATP and its metabolite adenosine regulate mucociliary clearance in airway epithelia. Little has been known, however, regarding the actual ATP and adenosine concentrations in the thin (
7 µm) liquid layer lining native airway surfaces and the link between ATP release/metabolism and autocrine/paracrine regulation of epithelial function. In this study, chimeric Staphylococcus aureus protein A-luciferase (SPA-luc) was bound to endogenous antigens on primary human bronchial epithelial (HBE) cell surface and ATP concentrations assessed in real-time in the thin airway surface liquid (ASL). ATP concentrations on resting cells were 1-10 nM. Inhibition of ecto-nucleotidases resulted in ATP accumulation at a rate of 250 fmol/min/cm2, reflecting the basal ATP release rate. Following hypotonic challenge to promote cell swelling, cell-surface ATP concentration measured by SPA-luc transiently reached 1 µM independent of ASL volume, reflecting a transient 3-log increase in ATP release rates. In contrast, peak ATP concentrations measured in bulk ASL by soluble luciferase inversely correlated with volume. ATP release rates were intracellular calcium-independent, suggesting that non-exocytotic ATP release from ciliated cells, which dominate our cultures, mediated hypotonicity-induced nucleotide release. However, the cystic fibrosis transmembrane conductance regulator (CFTR) did not participate in this function. Following the acute swelling phase, HBE cells exhibited regulatory volume decrease which was impaired by apyrase and facilitated by ATP or UTP. Our data provide the first evidence that ATP concentrations at the airway epithelial surface reach the range for P2Y2 receptor activation by physiological stimuli and identify a role for mucosal ATP release in airway epithelial cell volume regulation.
Received for publication, March 30, 2006 , and in revised form, June 2, 2006.
* This work was supported by the National Institutes of Health Grant NIH/NHLBI 5 P01 HL 34322-18 and Cystic Fibrosis Foundation Grant CFF R026-CR02 (both to R. C. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This article was selected as a Paper of the Week.
1 To whom correspondence should be addressed. Tel.: 919-966-7045; Fax: 919-966-5178; E-mail: seiko_okada{at}med.unc.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Zuo, M. Picher, S. F. Okada, E. R. Lazarowski, B. Button, R. C. Boucher, and T. C. Elston Mathematical Model of Nucleotide Regulation on Airway Epithelia: IMPLICATIONS FOR AIRWAY HOMEOSTASIS J. Biol. Chem., September 26, 2008; 283(39): 26805 - 26819. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Salin-Cantegrel, M. Shekarabi, S. Holbert, P. Dion, D. Rochefort, J. Laganiere, S. Dacal, P. Hince, L. Karemera, C. Gaspar, et al. HMSN/ACC truncation mutations disrupt brain-type creatine kinase-dependant activation of K+/Cl- co-transporter 3 Hum. Mol. Genet., September 1, 2008; 17(17): 2703 - 2711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Lorenzo, W. Liedtke, M. J. Sanderson, and M. A. Valverde TRPV4 channel participates in receptor-operated calcium entry and ciliary beat frequency regulation in mouse airway epithelial cells PNAS, August 26, 2008; 105(34): 12611 - 12616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Myrtek, T. Muller, V. Geyer, N. Derr, D. Ferrari, G. Zissel, T. Durk, S. Sorichter, W. Luttmann, M. Kuepper, et al. Activation of Human Alveolar Macrophages via P2 Receptors: Coupling to Intracellular Ca2+ Increases and Cytokine Secretion J. Immunol., August 1, 2008; 181(3): 2181 - 2188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Woo, A. K. Dutta, V. Patel, C. Kresge, and A. P. Feranchak Fluid flow induces mechanosensitive ATP release, calcium signalling and Cl- transport in biliary epithelial cells through a PKC{zeta}-dependent pathway J. Physiol., June 1, 2008; 586(11): 2779 - 2798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Y. Choi, J. Simon, K. W. K. Tsim, and E. A. Barnard Constitutive and Agonist-induced Dimerizations of the P2Y1 Receptor: RELATIONSHIP TO INTERNALIZATION AND SCAFFOLDING J. Biol. Chem., April 18, 2008; 283(16): 11050 - 11063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Fernandes, I. M. Lorenzo, Y. N. Andrade, A. Garcia-Elias, S. A. Serra, J. M. Fernandez-Fernandez, and M. A. Valverde IP3 sensitizes TRPV4 channel to the mechano- and osmotransducing messenger 5'-6'-epoxyeicosatrienoic acid J. Cell Biol., April 3, 2008; 181(1): 143 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Pafundo, O. Chara, M. P. Faillace, G. Krumschnabel, and P. J. Schwarzbaum Kinetics of ATP release and cell volume regulation of hyposmotically challenged goldfish hepatocytes Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R220 - R233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Vallon P2 receptors in the regulation of renal transport mechanisms Am J Physiol Renal Physiol, January 1, 2008; 294(1): F10 - F27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Akiba, M. Mizumori, P. H. Guth, E. Engel, and J. D. Kaunitz Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats Am J Physiol Gastrointest Liver Physiol, December 1, 2007; 293(6): G1223 - G1233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tatur, N. Groulx, S. N. Orlov, and R. Grygorczyk Ca2+-dependent ATP release from A549 cells involves synergistic autocrine stimulation by coreleased uridine nucleotides J. Physiol., October 15, 2007; 584(2): 419 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Kreda, S. F. Okada, C. A. van Heusden, W. O'Neal, S. Gabriel, L. Abdullah, C. W. Davis, R. C. Boucher, and E. R. Lazarowski Coordinated release of nucleotides and mucin from human airway epithelial Calu-3 cells J. Physiol., October 1, 2007; 584(1): 245 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Orlov and A. A. Mongin Salt-sensing mechanisms in blood pressure regulation and hypertension Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2039 - H2053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Button, M. Picher, and R. C. Boucher Differential effects of cyclic and constant stress on ATP release and mucociliary transport by human airway epithelia J. Physiol., April 15, 2007; 580(2): 577 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Schwiebert Extracellular autocrine nucleotide signalling in a microenvironment: integrative physiology in a minute volume of airway surface liquid J. Physiol., April 15, 2007; 580(2): 359 - 360. [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Winters, C. W. Davis, and R. C. Boucher Mechanosensitivity of mouse tracheal ciliary beat frequency: roles for Ca2+, purinergic signaling, tonicity, and viscosity Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L614 - L624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. V. Wesley, P. F. Bove, M. Hristova, S. McCarthy, and A. van der Vliet Airway Epithelial Cell Migration and Wound Repair by ATP-mediated Activation of Dual Oxidase 1 J. Biol. Chem., February 2, 2007; 282(5): 3213 - 3220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Reigada, W. Lu, and C. H. Mitchell Glutamate acts at NMDA receptors on fresh bovine and on cultured human retinal pigment epithelial cells to trigger release of ATP J. Physiol., September 15, 2006; 575(3): 707 - 720. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
