HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 33, 23579-23588, August 18, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/33/23579    most recent M602135200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arnaud, N. Articles by Gaymard, F. Search for Related Content PubMed PubMed Citation Articles by Arnaud, N. Articles by Gaymard, F. Social Bookmarking                      What's this?

An Iron-induced Nitric Oxide Burst Precedes Ubiquitin-dependent Protein Degradation for Arabidopsis AtFer1 Ferritin Gene Expression^*

Nicolas Arnaud¹, Irene Murgia, Jossia Boucherez, Jean-François Briat, Françoise Cellier, and Frédéric Gaymard²

From the Laboratoire de Biochimie et Physiologie Moléculaire des Plantes, UMR 5004 Agro-M/CNRS/INRA/UMII, Bat 7, 2 place Viala, 34060 Montpellier Cedex 1, France and the Sezione di Fisiologia e Biochimica delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, via Celoria 26, 20133 Milano, Italy

Ferritins play an essential role in iron homeostasis by sequestering iron in a bioavailable and non-toxic form. In plants, ferritin mRNAs are highly and quickly accumulated in response to iron overload. Such accumulation leads to a subsequent ferritin protein synthesis and iron storage, thus avoiding oxidative stress to take place. By combining pharmacological and imaging approaches in an Arabidopsis cell culture system, we have identified several elements in the signal transduction pathway leading to the increase of AtFer1 transcript level after iron treatment. Nitric oxide quickly accumulates in the plastids after iron treatment. This compound acts downstream of iron and upstream of a PP2A-type phosphatase to promote an increase of AtFer1 mRNA level. The AtFer1 gene transcription has been previously shown to be repressed under low iron conditions with the involvement of the cis-acting element iron-dependent regulatory sequence identified within the AtFer1 promoter sequence. We show here that the repressor is unlikely a transcription factor directly bound to the iron-dependent regulatory sequence; such a repressor is ubiquitinated upon iron treatment and subsequently degraded through a 26 S proteasome-dependent pathway.

Received for publication, March 7, 2006 , and in revised form, May 2, 2006.

^* This work was supported by Institut National de la Recherche Agronomique and Centre National de la Recherche Scientifique, Action Concertée Incitative "Biologie Cellulaire Moléculaire et Structurale" Grant BCMS166 from the Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Supported by a thesis fellowship from the Ministère de l'Education Nationale, de l'Enseignement Supérieur et de la Recherche.

² To whom correspondence should be addressed. Tel.: 33-499-61-29-32; Fax: 33-467-52-57-37; E-mail: gaymard{at}ensam.inra.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. W. Jin, S. T. Du, Y. S. Zhang, X. Y. Lin, and C. X. Tang Differential regulatory role of nitric oxide in mediating nitrate reductase activity in roots of tomato (Solanum lycocarpum) Ann. Bot., July 1, 2009; 104(1): 9 - 17. [Abstract] [Full Text] [PDF]

				L. Ramirez, E. J. Zabaleta, and L. Lamattina Nitric oxide and frataxin: two players contributing to maintain cellular iron homeostasis Ann. Bot., June 25, 2009; (2009) mcp147v1. [Abstract] [Full Text] [PDF]

				K. Ravet, B. Touraine, S. A. Kim, F. Cellier, S. Thomine, M. L. Guerinot, J.-F. Briat, and F. Gaymard Post-Translational Regulation of AtFER2 Ferritin in Response to Intracellular Iron Trafficking during Fruit Development in Arabidopsis Mol Plant, June 19, 2009; (2009) ssp041v1. [Abstract] [Full Text] [PDF]

				J.-F. Briat, K. Ravet, N. Arnaud, C. Duc, J. Boucherez, B. Touraine, F. Cellier, and F. Gaymard New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants Ann. Bot., May 29, 2009; (2009) mcp128v1. [Abstract] [Full Text] [PDF]

				A. Besson-Bard, A. Gravot, P. Richaud, P. Auroy, C. Duc, F. Gaymard, L. Taconnat, J.-P. Renou, A. Pugin, and D. Wendehenne Nitric Oxide Contributes to Cadmium Toxicity in Arabidopsis by Promoting Cadmium Accumulation in Roots and by Up-Regulating Genes Related to Iron Uptake Plant Physiology, March 1, 2009; 149(3): 1302 - 1315. [Abstract] [Full Text] [PDF]

				E. Gas, U. Flores-Perez, S. Sauret-Gueto, and M. Rodriguez-Concepcion Hunting for Plant Nitric Oxide Synthase Provides New Evidence of a Central Role for Plastids in Nitric Oxide Metabolism PLANT CELL, January 1, 2009; 21(1): 18 - 23. [Abstract] [Full Text] [PDF]

				M. Moreau, G. I. Lee, Y. Wang, B. R. Crane, and D. F. Klessig AtNOS/AtNOA1 Is a Functional Arabidopsis thaliana cGTPase and Not a Nitric-oxide Synthase J. Biol. Chem., November 21, 2008; 283(47): 32957 - 32967. [Abstract] [Full Text] [PDF]

				T. Flores, C. D. Todd, A. Tovar-Mendez, P. K. Dhanoa, N. Correa-Aragunde, M. E. Hoyos, D. M. Brownfield, R. T. Mullen, L. Lamattina, and J. C. Polacco Arginase-Negative Mutants of Arabidopsis Exhibit Increased Nitric Oxide Signaling in Root Development Plant Physiology, August 1, 2008; 147(4): 1936 - 1946. [Abstract] [Full Text] [PDF]

				A. Besson-Bard, C. Courtois, A. Gauthier, J. Dahan, G. Dobrowolska, S. Jeandroz, A. Pugin, and D. Wendehenne Nitric Oxide in Plants: Production and Cross-talk with Ca2+ Signaling Mol Plant, March 1, 2008; 1(2): 218 - 228. [Abstract] [Full Text] [PDF]

				U. Lee, C. Wie, B. O. Fernandez, M. Feelisch, and E. Vierling Modulation of Nitrosative Stress by S-Nitrosoglutathione Reductase Is Critical for Thermotolerance and Plant Growth in Arabidopsis PLANT CELL, March 1, 2008; 20(3): 786 - 802. [Abstract] [Full Text] [PDF]

				S. Neill, R. Barros, J. Bright, R. Desikan, J. Hancock, J. Harrison, P. Morris, D. Ribeiro, and I. Wilson Nitric oxide, stomatal closure, and abiotic stress J. Exp. Bot., February 1, 2008; 59(2): 165 - 176. [Abstract] [Full Text] [PDF]

				S. J. Romney, C. Thacker, and E. A. Leibold An Iron Enhancer Element in the FTN-1 Gene Directs Iron-dependent Expression in Caenorhabditis elegans Intestine J. Biol. Chem., January 11, 2008; 283(2): 716 - 725. [Abstract] [Full Text] [PDF]

				S. Neill, J. Bright, R. Desikan, J. Hancock, J. Harrison, and I. Wilson Nitric oxide evolution and perception J. Exp. Bot., January 1, 2008; 59(1): 25 - 35. [Abstract] [Full Text] [PDF]

				S. Frigerio, C. Campoli, S. Zorzan, L. I. Fantoni, C. Crosatti, F. Drepper, W. Haehnel, L. Cattivelli, T. Morosinotto, and R. Bassi Photosynthetic Antenna Size in Higher Plants Is Controlled by the Plastoquinone Redox State at the Post-transcriptional Rather than Transcriptional Level J. Biol. Chem., October 5, 2007; 282(40): 29457 - 29469. [Abstract] [Full Text] [PDF]