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J. Biol. Chem., Vol. 281, Issue 35, 25475-25484, September 1, 2006

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The C Terminus of the Immunophilin PASTICCINO1 Is Required for Plant Development and for Interaction with a NAC-like Transcription Factor^*

Cybelle Smyczynski¹, François Roudier², Lionel Gissot, Emilie Vaillant, Olivier Grandjean, Halima Morin, Thimoté Masson, Yannick Bellec, Danny Geelen^¶3, and Jean-Denis Faure⁴

From the Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, Route de St. Cyr, 78026 Versailles Cedex, France, Laboratoire Commun de Cytologie, Institut Jean-Pierre Bourgin, INRA, Route de St. Cyr, 78026 Versailles Cedex, France, and ^¶Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, Technologiepark 927, B-9052 Ghent, Belgium

PASTICCINO1 (PAS1) is a high molecular weight FK506-binding protein (FKBP) involved in the control of cell proliferation and differentiation during plant development. Mutations in the C-terminal region of PAS1 result in severe developmental defects. We show here that the C-terminal domain of PAS1 controls the subcellular distribution of this protein. We also demonstrated in vitro and in vivo, by Forster resonance energy transfer, that this C-terminal region is required for interaction with FAN (FKBP-associated NAC), a new member of the plant-specific family of NAC transcription factors. PAS1 and FAN are translocated into the nucleus upon auxin treatment in plant seedlings. The nuclear translocation of PAS1 is dependent on the presence of the C terminus of the protein. Finally, we showed that FAN is involved in PAS1-regulated processes because FAN overproduction partly complemented the pas1 phenotype. We suggest that PAS1 regulates the function of this NAC-like transcription factor by controlling its targeting to the nucleus upon plant cell division.

Received for publication, February 24, 2006 , and in revised form, June 22, 2006.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Fig. 1.

¹ Supported by Action Concertée Incitative "Jeune Chercheur" 5091 and Génoplante Programme "Nouveau Outils" NO2001043. Present address: London School of Hygiene and Tropical Medicine, Keppel St., London WC1E 7HT, UK.

² Recipient of a postdoctoral grant from INRA. Present address: Unité de Recherche en Génomique Végétale, 2 Rue Gaston Crémieux, CP 5708, 91057 EVRY Cedex, France.

³ Present address: Dept. of Plant Production, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.

⁴ To whom correspondence should be addressed. Tel.: 33-1-30-83-31-13; Fax: 33-1-30-83-30-99; E-mail: faure{at}versailles.inra.fr.

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