HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 35, 25791-25802, September 1, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/35/25791    most recent M604501200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, Y. Articles by Bogenhagen, D. F. Search for Related Content PubMed PubMed Citation Articles by Wang, Y. Articles by Bogenhagen, D. F. Social Bookmarking                      What's this?

Human Mitochondrial DNA Nucleoids Are Linked to Protein Folding Machinery and Metabolic Enzymes at the Mitochondrial Inner Membrane^*

From the Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794-8651

Mitochondrial DNA (mtDNA) is packaged into bacterial nucleoid-like structures, each containing several mtDNA molecules. The distribution of nucleoids during mitochondrial fission and fusion events and during cytokinesis is important to the segregation of mitochondrial genomes in heteroplasmic cells bearing a mixture of wild-type and mutant mtDNA molecules. We report fractionation of HeLa cell mtDNA nucleoids into two subsets of complexes that differ in their sedimentation velocity and their association with cytoskeletal proteins. Pulse labeling studies indicated that newly replicated mtDNA molecules are evenly represented in the rapidly and slowly sedimenting fractions. Slowly sedimenting nucleoids were immunoaffinity purified using antibodies to either of two abundant mtDNA-binding proteins, TFAM or mtSSB. These two different immunoaffinity procedures yielded very similar sets of proteins, with 21 proteins in common, including most of the proteins previously shown to play roles in mtDNA replication and transcription. In addition to previously identified mitochondrial proteins, multiple peptides were observed for one novel DNA metabolic protein, the DEAH-box helicase DHX30. Antibodies raised against a recombinant fragment of this protein confirmed the mitochondrial localization of a specific isoform of DHX30.

Received for publication, May 10, 2006 , and in revised form, June 13, 2006.

^* This work was supported by National Institutes of Health Grants R01GM29681 and R01ES012039 (to D. F. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental materials, Figs. S1-S3, and a table.

¹ To whom correspondence should be addressed. Tel.: 631-444-3068; Fax: 631-444-3218; E-mail: dan{at}pharm.sunysb.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Kienhofer, D. J. F. Haussler, F. Ruckelshausen, E. Muessig, K. Weber, D. Pimentel, V. Ullrich, A. Burkle, and M. M. Bachschmid Association of mitochondrial antioxidant enzymes with mitochondrial DNA as integral nucleoid constituents FASEB J, July 1, 2009; 23(7): 2034 - 2044. [Abstract] [Full Text] [PDF]

				I. Miyakawa, A. Okamuro, S. Kinsky, K. Visacka, L. Tomaska, and J. Nosek Mitochondrial nucleoids from the yeast Candida parapsilosis: expansion of the repertoire of proteins associated with mitochondrial DNA Microbiology, May 1, 2009; 155(5): 1558 - 1568. [Abstract] [Full Text] [PDF]

				T. S. Wong, S. Rajagopalan, F. M. Townsley, S. M. Freund, M. Petrovich, D. Loakes, and A. R. Fersht Physical and functional interactions between human mitochondrial single-stranded DNA-binding protein and tumour suppressor p53 Nucleic Acids Res., February 1, 2009; 37(2): 568 - 581. [Abstract] [Full Text] [PDF]

				M. Iacovino, C. Granycome, H. Sembongi, M. Bokori-Brown, R. A. Butow, I. J. Holt, and J. M. Bateman The conserved translocase Tim17 prevents mitochondrial DNA loss Hum. Mol. Genet., January 1, 2009; 18(1): 65 - 74. [Abstract] [Full Text] [PDF]

				J.-Y. Song, K.-D. Kim, and J.-H. Roe Thiol-Independent Action of Mitochondrial Thioredoxin To Support the Urea Cycle of Arginine Biosynthesis in Schizosaccharomyces pombe Eukaryot. Cell, December 1, 2008; 7(12): 2160 - 2167. [Abstract] [Full Text] [PDF]

				R. W. Gilkerson, E. A. Schon, E. Hernandez, and M. M. Davidson Mitochondrial nucleoids maintain genetic autonomy but allow for functional complementation J. Cell Biol., October 22, 2008; 181(7): 1117 - 1128. [Abstract] [Full Text] [PDF]

				L. Khidr, G. Wu, A. Davila, V. Procaccio, D. Wallace, and W.-H. Lee Role of SUV3 Helicase in Maintaining Mitochondrial Homeostasis in Human Cells J. Biol. Chem., October 3, 2008; 283(40): 27064 - 27073. [Abstract] [Full Text] [PDF]

				J. Rorbach, R. Richter, H. J. Wessels, M. Wydro, M. Pekalski, M. Farhoud, I. Kuhl, M. Gaisne, N. Bonnefoy, J. A. Smeitink, et al. The human mitochondrial ribosome recycling factor is essential for cell viability Nucleic Acids Res., October 1, 2008; 36(18): 5787 - 5799. [Abstract] [Full Text] [PDF]

				M. Kucej, B. Kucejova, R. Subramanian, X. J. Chen, and R. A. Butow Mitochondrial nucleoids undergo remodeling in response to metabolic cues J. Cell Sci., June 1, 2008; 121(11): 1861 - 1868. [Abstract] [Full Text] [PDF]

				R. C. Scarpulla Transcriptional Paradigms in Mammalian Mitochondrial Biogenesis and Function Physiol Rev, April 1, 2008; 88(2): 611 - 638. [Abstract] [Full Text] [PDF]

				S.-H. Chen, C. K. Suzuki, and S.-H. Wu Thermodynamic characterization of specific interactions between the human Lon protease and G-quartet DNA Nucleic Acids Res., March 27, 2008; 36(4): 1273 - 1287. [Abstract] [Full Text] [PDF]

				D. F. Bogenhagen, D. Rousseau, and S. Burke The Layered Structure of Human Mitochondrial DNA Nucleoids J. Biol. Chem., February 8, 2008; 283(6): 3665 - 3675. [Abstract] [Full Text] [PDF]

				M. Zeviani OPA1 mutations and mitochondrial DNA damage: keeping the magic circle in shape Brain, February 1, 2008; 131(2): 314 - 317. [Full Text] [PDF]

				B. A. Kaufman, N. Durisic, J. M. Mativetsky, S. Costantino, M. A. Hancock, P. Grutter, and E. A. Shoubridge The Mitochondrial Transcription Factor TFAM Coordinates the Assembly of Multiple DNA Molecules into Nucleoid-like Structures Mol. Biol. Cell, September 1, 2007; 18(9): 3225 - 3236. [Abstract] [Full Text] [PDF]

				X. J. Chen, X. Wang, and R. A. Butow Yeast aconitase binds and provides metabolically coupled protection to mitochondrial DNA PNAS, August 21, 2007; 104(34): 13738 - 13743. [Abstract] [Full Text] [PDF]

				J. He, C.-C. Mao, A. Reyes, H. Sembongi, M. Di Re, C. Granycome, A. B. Clippingdale, I. M. Fearnley, M. Harbour, A. J. Robinson, et al. The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization J. Cell Biol., January 16, 2007; 176(2): 141 - 146. [Abstract] [Full Text] [PDF]