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J. Biol. Chem., Vol. 281, Issue 37, 26922-26931, September 15, 2006

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Dinucleotide Spore Photoproduct, a Minimal Substrate of the DNA Repair Spore Photoproduct Lyase Enzyme from Bacillus subtilis^*

Alexia Chandor¹², Olivier Berteau¹³, Thierry Douki, Didier Gasparutto, Yannis Sanakis^¶, Sandrine Ollagnier-de-Choudens, Mohamed Atta, and Marc Fontecave⁴

From the Laboratoire de Chimie et Biochimie des Centres Rédox Biologiques, DRDC-CB, UMR 5047, Commissariat à l'Energie Atomique/CNRS/Université Joseph Fourier, 17 Rue des Martyrs 38054, Grenoble Cedex 09, France, Service de Chimie Inorganique et Biologique UMR-E3, 5047 Commissariat à l'Energie Atomique/Université Joseph Fourier, Département de Recherche Fondamentale sur la Matière Condensée, 17 Rue des Martyrs, 38054 Grenoble Cedex 09, France, and ^¶NCSR Demokritos, Institute of Materials Science, Ag. Paraskevi, 15310 Attiki, Greece

The overwhelming majority of DNA photoproducts in UV-irradiated spores is a unique thymine dimer called spore photoproduct (SP, 5-thymine-5,6-dihydrothymine). This lesion is repaired by the spore photoproduct lyase (SP lyase) enzyme that directly reverts SP to two unmodified thymines. The SP lyase is an S-adenosylmethionine-dependent iron-sulfur protein that belongs to the radical S-adenosylmethionine superfamily. In this study, by using a well characterized preparation of the SP lyase enzyme from Bacillus subtilis, we show that SP in the form of a dinucleoside monophosphate (spore photoproduct of thymidilyl-(3'–5')-thymidine) is efficiently repaired, allowing a kinetic characterization of the enzyme. The preparation of this new substrate is described, and its identity is confirmed by mass spectrometry and comparison with authentic spore photoproduct. The fact that the spore photoproduct of thymidilyl-(3'–5')-thymidine dimer is repaired by SP lyase may indicate that the SP lesion does not absolutely need to be contained within a single- or double-stranded DNA for recognition and repaired by the SP lyase enzyme.

Received for publication, March 10, 2006 , and in revised form, June 23, 2006.

^* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Both authors contributed equally to this work.

² Supported by a Ph.D. fellowship grant from the Commissariat à l'Energie Atomique.

³ Supported by a postdoctoral fellowship grant from the Commissariat à l'Energie Atomique.

⁴ To whom correspondence should be addressed. Tel.: 33-4-38-78-91-03; Fax: 33-4-38-78-91-24; E-mail: marc.fontecave{at}cea.fr.

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