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J. Biol. Chem., Vol. 281, Issue 38, 27806-27815, September 22, 2006

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Escherichia coli Transcriptome Dynamics during the Transition from Anaerobic to Aerobic Conditions^*

From the Department of Molecular Biology and Biotechnology, the University of Sheffield, Sheffield S10 2TN, United Kingdom

Escherichia coli is a metabolically versatile bacterium that is able to grow in the presence and absence of oxygen. Several previous transcript-profiling experiments have compared separate anaerobic and aerobic cultures. Here the process of adaptation was investigated by determining changes in transcript profiles when anaerobic steady-state cultures were perturbed by the introduction of air. Within 5 min of culture aeration the abundances of transcripts associated with anaerobic metabolism were decreased, whereas transcripts associated with aerobic metabolism were increased. In addition to the rapid switch to aerobic central metabolism, transcript profiling, supported by experiments with relevant mutants, revealed transient changes suggesting that the peroxide stress response, methionine biosynthesis, and degradation of putrescine play important roles during the adaptation to aerobic conditions.

Received for publication, April 11, 2006 , and in revised form, June 27, 2006.

^* This work was supported by the UK Biotechnology and Biological Sciences Research Council. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Figs. 1–3 and Tables I–IV.

¹ To whom correspondence should be addressed. Tel.: 44-114-222-4403; Fax: 44-114-222-2800; E-mail: jeff.green{at}sheffield.ac.uk.

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