HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 39, 28910-28918, September 29, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/39/28910    most recent M606092200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zanzouri, M. Articles by Patel, A. Search for Related Content PubMed PubMed Citation Articles by Zanzouri, M. Articles by Patel, A. Social Bookmarking                      What's this?

Membrane Potential-regulated Transcription of the Resting K⁺ Conductance TASK-3 via the Calcineurin Pathway^*

From the Institut de Pharmacologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, UMR 6097, Université de Nice-Sophia Antipolis, Valbonne 06560, France

The 2P domain K⁺ channel TASK-3 is highly expressed in cerebellar granule neurons where it has been proposed to underlie the K⁺ leak conductance, IKso. In a previous work we showed that expression of TASK-3 increases in cerebellar granule neurons as they mature in culture. Here we show that within the cerebellum, levels of TASK-3 mRNA increase as granule neurons migrate to their adult positions and receive excitatory mossy fiber input. To understand the mechanism of this increase in TASK-3 expression we used an in vitro model culturing the neurons in either depolarizing conditions mimicking neuronal activity (25K, 25 mM KCl) or in conditions which approach deafferentation (5K, 5 mM KCl). An important increase in TASK-3 mRNA is uniquely observed in 25K and is specific since other background K⁺ channel levels remain unchanged or decrease. The rise in TASK-3 mRNA leads to an increase in TASK-3 protein and the IKso conductance resulting in hyperpolarization. Blocking L-type calcium channels or their downstream effector calcineurin, abrogates TASK-3 expression and IKso, leading to hyperexcitability. This is the first study demonstrating that depolarization-induced Ca²⁺ entry can directly regulate cellular excitability by dynamically regulating the transcription of a resting K⁺ conductance. The appearance of this conductance may play an important role in the transition of depolarized immature neurons to their mature hyperpolarized state during neuronal development.

Received for publication, June 26, 2006 , and in revised form, July 24, 2006.

^* This work was funded by La Ligue Nationale contre le Cancer (Equipe labelisé) and Association Pour La Recherche Sur le Cancer (Subvention number 3331) and the Institute Paul Hamel and by the CNRS. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental primer information and Fig.1.

¹ These authors contributed equally to this work.

² To whom correspondence should be addressed: IPMC-CNRS, UMR6097, 660 Route des Lucioles, Sophia Antipolis, 06560 Valbonne, France. Tel.: 33-493957730; Fax: 33-493957704; E-mail: patel{at}ipmc.cnrs.fr.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Okazawa, H. Abe, M. Katsukawa, K. Iijima, T. Kiwada, and S. Nakanishi Role of Calcineurin Signaling in Membrane Potential-Regulated Maturation of Cerebellar Granule Cells J. Neurosci., March 4, 2009; 29(9): 2938 - 2947. [Abstract] [Full Text] [PDF]

				K. K. Kunjilwar, H. M. Fishman, D. J. Englot, R. G. O'Neil, and E. T. Walters Long-Lasting Hyperexcitability Induced by Depolarization in the Absence of Detectable Ca2+ Signals J Neurophysiol, March 1, 2009; 101(3): 1351 - 1360. [Abstract] [Full Text] [PDF]

				M. Zuzarte, K. Heusser, V. Renigunta, G. Schlichthorl, S. Rinne, E. Wischmeyer, J. Daut, B. Schwappach, and R. Preisig-Muller Intracellular traffic of the K+ channels TASK-1 and TASK-3: role of N- and C-terminal sorting signals and interaction with 14-3-3 proteins J. Physiol., March 1, 2009; 587(5): 929 - 952. [Abstract] [Full Text] [PDF]

				A. Guyon, M. P. Tardy, C. Rovere, J.-L. Nahon, J. Barhanin, and F. Lesage Glucose Inhibition Persists in Hypothalamic Neurons Lacking Tandem-Pore K+ Channels J. Neurosci., February 25, 2009; 29(8): 2528 - 2533. [Abstract] [Full Text] [PDF]

				X. Liu and C. Chen Different Roles for AMPA and NMDA Receptors in Transmission at the Immature Retinogeniculate Synapse J Neurophysiol, February 1, 2008; 99(2): 629 - 643. [Abstract] [Full Text] [PDF]

				T. Dobler, A. Springauf, S. Tovornik, M. Weber, A. Schmitt, R. Sedlmeier, E. Wischmeyer, and F. Doring TRESK two-pore-domain K+ channels constitute a significant component of background potassium currents in murine dorsal root ganglion neurones J. Physiol., December 15, 2007; 585(3): 867 - 879. [Abstract] [Full Text] [PDF]

				N. Ruf, S. Bahring, D. Galetzka, G. Pliushch, F. C. Luft, P. Nurnberg, T. Haaf, G. Kelsey, and U. Zechner Sequence-based bioinformatic prediction and QUASEP identify genomic imprinting of the KCNK9 potassium channel gene in mouse and human Hum. Mol. Genet., November 1, 2007; 16(21): 2591 - 2599. [Abstract] [Full Text] [PDF]

				S. G. Brickley, M. I. Aller, C. Sandu, E. L. Veale, F. G. Alder, H. Sambi, A. Mathie, and W. Wisden TASK-3 Two-Pore Domain Potassium Channels Enable Sustained High-Frequency Firing in Cerebellar Granule Neurons J. Neurosci., August 29, 2007; 27(35): 9329 - 9340. [Abstract] [Full Text] [PDF]