HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 45, 33860-33870, November 10, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/45/33860    most recent M605905200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hochman, E. Articles by Izraeli, S. Search for Related Content PubMed PubMed Citation Articles by Hochman, E. Articles by Izraeli, S. Social Bookmarking                      What's this?

Molecular Pathways Regulating Pro-migratory Effects of Hedgehog Signaling^*

From the Research Section of Childhood Malignancies, Sheba Cancer Research Center, Safra Children Hospital, Sheba Medical Center and Faculty of Medicine, Tel-Aviv University, Tel Hashomer 52621, Israel

The Hedgehog proteins play a crucial role in metazoan embryo development. Constitutive activation of the pathway is associated with multiple types of cancer. Recent experimental data suggest involvement of Hedgehog signaling in vascular remodeling, germ cell migration, and axon guidance. The molecular mechanisms underlying these effects remain elusive. Here we show that yolk sac-derived endothelial cells and embryonic fibroblasts can directly respond to the Hedgehog signal by increased migration in an in vitro scratch (wound) assay. We also identify Hedgehog transcriptional target genes in these cells, many of which participate in cell migration, axon guidance, and angiogenesis processes. Inhibition of one such molecular pathway, neuropilin-flavomonooxygenase, blocks Hedgehog-induced cell migration. These findings suggest that Hedgehog signaling directly affects embryonic endothelial and fibroblast cell migration via molecules and pathways known to regulate cell migration in response to a variety of environmental cues.

Received for publication, June 20, 2006 , and in revised form, August 29, 2006.

^* This work was supported in part by the Israel Science Foundation, The Leukemia Research Foundation, The Israel Cancer Research Foundation, and The Recannati Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Tables 1 and 2 and supplemental Figs. 1 and 2.

¹ Performed this work in partial fulfillment of the requirements for the M.D.-Ph.D. thesis at Sackler Faculty of Medicine, Tel Aviv University, Israel.

² To whom correspondence should be addressed. E-mail: sizraeli{at}sheba.health.gov.il.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y. A. Yoo, M. H. Kang, J. S. Kim, and S. C. Oh Sonic hedgehog signaling promotes motility and invasiveness of gastric cancer cells through TGF-{beta}-mediated activation of the ALK5-Smad 3 pathway Carcinogenesis, March 1, 2008; 29(3): 480 - 490. [Abstract] [Full Text] [PDF]