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J. Biol. Chem., Vol. 281, Issue 49, 37636-37645, December 8, 2006

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A R2R3 Type MYB Transcription Factor Is Involved in the Cold Regulation of CBF Genes and in Acquired Freezing Tolerance^*

From the Institute for Integrative Genome Biology and Department of Botany & Plant Science, University of California, Riverside, California 92521

Cold temperatures trigger the expression of the CBF family of transcription factors, which in turn activate many downstream genes that confer freezing tolerance to plants. It has been shown previously that the cold regulation of CBF3 involves an upstream bHLH-type transcription factor, ICE1. ICE1 binds to the Myc recognition sequences in the CBF3 promoter. Apart from Myc recognition sequences, CBF promoters also have Myb recognition sequences. We report here that the Arabidopsis MYB15 is involved in cold-regulation of CBF genes and in the development of freezing tolerance. The MYB15 gene transcript is up-regulated by cold stress. The MYB15 protein interacts with ICE1 and binds to Myb recognition sequences in the promoters of CBF genes. Overexpression of MYB15 results in reduced expression of CBF genes whereas its loss-of-function leads to increased expression of CBF genes in the cold. The myb15 mutant plants show increased tolerance to freezing stress whereas its overexpression reduces freezing tolerance. Our results suggest that MYB15 is part of a complex network of transcription factors controlling the expression of CBFs and other genes in response to cold stress.

Received for publication, June 20, 2006 , and in revised form, September 29, 2006.

^* This work was supported in part by National Science Foundation MCB-0241450 and United States Department of Agriculture NRI 2003-00751 and National Institutes of Health Grant R01 GM059138 (to J.-K. Z.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Botany and Plant Sciences, 2150 Batchelor Hall, University of California, Riverside, CA 92521. Tel.: 951-827-7117; Fax: 951-827-7115; E-mail: jian-kang.zhu{at}ucr.edu.

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