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J. Biol. Chem., Vol. 281, Issue 5, 2828-2834, February 3, 2006

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Dual Role of the Cdc7-regulatory Protein Dbf4 during Yeast Meiosis^*

From the Institute of Molecular Genetics and Université Montpellier II, CNRS UMR5535-1919, Route de Mende, F-34293 Montpellier Cedex 5, France

The Dbf4-dependent Cdc7 kinase (DDK) is essential for chromosome duplication in all eukaryotes, but was proposed to be dispensable for yeast pre-meiotic DNA replication. This discrepancy led us to investigate the role of the unstable Cdc7-regulatory protein Dbf4 in meiosis. We show that, when Dbf4 is depleted at the time of meiotic induction, cells enter the meiotic program but do not replicate their chromosomes. Surprisingly when Dbf4 is depleted after the initiation of DNA synthesis, S phase goes to completion, but most cells arrest before anaphase I. Deletion of the cohesin Rec8 suppresses this phenotype, suggesting a distinct role of DDK for meiotic chromosome segregation. As after Cdc5 depletion, a fraction of cells undergo a single equational division suggesting a failure to mono-orient sister kinetochores. Our results demonstrate that Dbf4 is essential for DNA replication during meiosis like in vegetative cells and provide evidence for an additional role in setting up the reductional division of meiosis I.

Received for publication, September 28, 2005 , and in revised form, November 29, 2005.

^* This work was financed through grants from the French Ministry of Research (ACI-BCMS no. 0230) and Association pour la Recherche sur le Cancer (ARC no. 4704) (to E. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1 and S2.

¹ To whom correspondence may be addressed. Tel.: 33-467-61-36-77; Fax: 33-467-04-02-31; E-mail: etienne.schwob{at}igmm.cnrs.fr.

² To whom correspondence may be addressed. Tel.: 33-467-61-36-77; Fax: 33-467-04-0231; E-mail: flavio.dellaseta{at}igmm.fr.

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