HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 50, 38609-38616, December 15, 2006

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 281/50/38609    most recent M512847200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, X.-W. Articles by Saltiel, A. R. Search for Related Content PubMed PubMed Citation Articles by Chen, X.-W. Articles by Saltiel, A. R. Social Bookmarking                      What's this?

RalA-exocyst-dependent Recycling Endosome Trafficking Is Required for the Completion of Cytokinesis^*

Xiao-Wei Chen, Mayumi Inoue, Shu C. Hsu^¶, and Alan R. Saltiel¹

From the Department of Molecular and Integrative Physiology, University of Michigan Medical Center, Ann Arbor, Michigan 48109, the Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan 48109, and the ^¶Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854

In eukaryotic cells, recycling endosome-mediated trafficking contributes to the completion of cytokinesis, in a manner under the control of the centrosome. We report that the exocyst complex and its interacting GTPase RalA play a critical role in this polarized trafficking process. RalA resides in the recycling endosome and relocates from the pericentrosomal region to key cytokinetic structures including the cleavage furrow, and later, the abscission site. This event is coupled to the dynamic redistribution of the exocyst proteins. These associate with the centrosome in interphase and concentrate on the central spindle/midbody during cytokinesis. Disruption of RalA-exocyst function leads to cytokinesis failure in late stages, particularly abscission, resembling the cytokinesis defects induced by loss of centrosome function. These data suggest that RalA and the exocyst may regulate vesicle delivery to the centrosome-related abscission site during the terminal stage of cytokinesis, implicating RalA as a critical regulator of cell cycle progression.

Received for publication, December 1, 2005 , and in revised form, July 20, 2006.

^* This work was supported by National Institutes of Health Grant R01DK061618 (to A. R. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains three supplemental figures.

¹ To whom correspondence should be addressed. Tel.: 734-615-9787; E-mail: saltiel{at}umich.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. W. Goss and D. K. Toomre Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis J. Cell Biol., October 22, 2008; 181(7): 1047 - 1054. [Abstract] [Full Text] [PDF]

				S. C. Falsetti, D.-a. Wang, H. Peng, D. Carrico, A. D. Cox, C. J. Der, A. D. Hamilton, and S. M. Sebti Geranylgeranyltransferase I Inhibitors Target RalB To Inhibit Anchorage-Dependent Growth and Induce Apoptosis and RalA To Inhibit Anchorage-Independent Growth Mol. Cell. Biol., November 15, 2007; 27(22): 8003 - 8014. [Abstract] [Full Text] [PDF]