HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 51, 38974-38980, December 22, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/51/38974    most recent M605370200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ooi, L. Articles by Buckley, N. J. Search for Related Content PubMed PubMed Citation Articles by Ooi, L. Articles by Buckley, N. J. Social Bookmarking                      What's this?

BRG1 Chromatin Remodeling Activity Is Required for Efficient Chromatin Binding by Repressor Element 1-silencing Transcription Factor (REST) and Facilitates REST-mediated Repression^*

Lezanne Ooi¹, Nikolai D. Belyaev, Katsuhide Miyake, Ian C. Wood, and Noel J. Buckley²

From the Institute of Membrane and Systems Biology, University of Leeds, Leeds LS2 9JT, United Kingdom, and the Department of Biotechnology, Nagoya University, Nagoya 464-8603, Japan

Chromatin remodeling enzymes such as SWI/SNF use the hydrolysis of ATP to power the movement of nucleosomes with respect to DNA. BRG1, one of the ATPases of the SWI/SNF complex, can be recruited by both activators and repressors, although the precise role of BRG1 in mechanisms of repression has thus far remained unclear. One transcription factor that recruits BRG1 as a corepressor is the repressor element 1-silencing transcription factor (REST). Here we address for the first time the mechanism of BRG1 activity in gene repression. We found that BRG1 enhanced REST-mediated repression at some REST target genes by increasing the interaction of REST with the local chromatin at its binding sites. Furthermore, REST-chromatin interactions, mediated by BRG1, were enhanced following an increase in histone acetylation in a manner dependent on the BRG1 bromodomain. Our data suggest that BRG1 facilitates REST repression by increasing the interaction between REST and chromatin. Such a mechanism may be applicable to other transcriptional repressors that utilize BRG1.

Received for publication, June 5, 2006 , and in revised form, September 26, 2006.

^* This work was supported by the Wellcome Trust. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

² Current address: Centre for the Cellular Basis of Behaviour, King's College London, Inst. of Psychiatry, De Crespigny Park, Box PO37, Denmark Hill, London SE5 8AF, United Kingdom.

¹ Supported by a Wellcome Trust Prize Ph.D. studentship. To whom correspondence should be addressed: Inst. of Membrane and Systems Biology, Garstang Bldg., University of Leeds, Mount Preston St., Leeds, LS2 9JT, United Kingdom. Tel.: 44-113-3437921; Fax: 44-113-3434228; E-mail: L.Ooi{at}leeds.ac.uk.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. Johnson, J. Samuel, C. K. L. Ng, R. Jauch, L. W. Stanton, and I. C. Wood Evolution of the Vertebrate Gene Regulatory Network Controlled by the Transcriptional Repressor REST Mol. Biol. Evol., July 1, 2009; 26(7): 1491 - 1507. [Abstract] [Full Text] [PDF]

				M. D. Kaeser, A. Aslanian, M.-Q. Dong, J. R. Yates III, and B. M. Emerson BRD7, a Novel PBAF-specific SWI/SNF Subunit, Is Required for Target Gene Activation and Repression in Embryonic Stem Cells J. Biol. Chem., November 21, 2008; 283(47): 32254 - 32263. [Abstract] [Full Text] [PDF]

				X. Tang, A. Hou, M. Babu, V. Nguyen, L. Hurtado, Q. Lu, J. C. Reyes, A. Wang, W. A. Keller, J. J. Harada, et al. The Arabidopsis BRAHMA Chromatin-Remodeling ATPase Is Involved in Repression of Seed Maturation Genes in Leaves Plant Physiology, July 1, 2008; 147(3): 1143 - 1157. [Abstract] [Full Text] [PDF]

				L. Abramovitz, T. Shapira, I. Ben-Dror, V. Dror, L. Granot, T. Rousso, E. Landoy, L. Blau, G. Thiel, and L. Vardimon Dual Role of NRSF/REST in Activation and Repression of the Glucocorticoid Response J. Biol. Chem., January 4, 2008; 283(1): 110 - 119. [Abstract] [Full Text] [PDF]

				R. W. Gunawardena, S. R. Fox, H. Siddiqui, and E. S. Knudsen SWI/SNF Activity Is Required for the Repression of Deoxyribonucleotide Triphosphate Metabolic Enzymes via the Recruitment of mSin3B J. Biol. Chem., July 13, 2007; 282(28): 20116 - 20123. [Abstract] [Full Text] [PDF]

				A. J. Bingham, L. Ooi, L. Kozera, E. White, and I. C. Wood The Repressor Element 1-Silencing Transcription Factor Regulates Heart-Specific Gene Expression Using Multiple Chromatin-Modifying Complexes Mol. Cell. Biol., June 1, 2007; 27(11): 4082 - 4092. [Abstract] [Full Text] [PDF]