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J. Biol. Chem., Vol. 281, Issue 6, 3343-3353, February 10, 2006

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Modulation of Neural Carbohydrate Epitope Expression in Drosophila melanogaster Cells^*

Dubravko Rendi¹, Angela Linder, Katharina Paschinger, Nicole Borth, Iain B. H. Wilson, and Gustáv Fabini²

From the Department für Chemie and Department für Biotechnologie, Universität für Bodenkultur, Muthgasse 18, Wien A-1190, Austria

Neural pathways in invertebrates are often tracked using anti-horseradish peroxidase, a cross-reaction due to the presence of core 1,3-fucosylated N-glycans. In order to investigate the molecular basis of this epitope in a cellular context, we compared two Drosophila melanogaster cell lines: the S2 and the neuronal-like BG2-c6 cell lines. As shown by mass spectrometric and chromatographic analyses, only the BG2-c6 cell line expresses 1,3/1,6-difucosylated N-glycans, a result that correlates with anti-horseradish peroxidase binding. Of all four 1,3-fucosyltransferase homologues previously identified, the core 1,3-fucosyltransferase (FucTA; EC 2.4.1.214 [EC] ) is expressed in the neuronal cell line as well as throughout fly development and in heads and bodies of flies of both sexes. This pattern is distinctive in comparison with the expression of the other three 1,3-fucosyltransferase homologues (FucTB, FucTC, and FucTD). Furthermore, only transfection of FucTA cDNA into S2 cells resulted in expression of the anti-horseradish peroxidase epitope, a result compatible with its substrate specificity in vitro. Finally, silencing of FucTA by RNAi in the neuronal cell line led to a significant reduction of anti-horseradish peroxidase binding. The present study, in conjunction with our previous in vitro data, thereby shows that FucTA is indispensable for expression of the neural carbohydrate epitope in Drosophila cells.

Received for publication, July 29, 2005 , and in revised form, November 22, 2005.

^* This work was funded by Fonds zur Förderung der Wissenschaftlichen Forschung Grants P13810 [GenBank] and P17681 [GenBank] (to I. B. H. W.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

² Present address: Octapharma Pharmazeutika Produktionsges.m.b.H., A-1100, Wien, Austria.

¹ To whom correspondence should be addressed. Tel.: 43-1-36006-6065; Fax: 43-1-36006-6059; E-mail: dubravko.rendic{at}boku.ac.at.

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