HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 281, Issue 9, 6087-6095, March 3, 2006

This Article Full Text Full Text (PDF) All Versions of this Article: 281/9/6087    most recent M507610200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lu, G. Articles by Wang, Y. Search for Related Content PubMed PubMed Citation Articles by Lu, G. Articles by Wang, Y. Social Bookmarking                      What's this?

TAB-1 Modulates Intracellular Localization of p38 MAP Kinase and Downstream Signaling^*

Gang Lu, Young Jun Kang^¶, Jiahuai Han^¶, Harvey R. Herschman^||, Enrico Stefani, and Yibin Wang, An Established Investigator of the American Heart Association¹

From the Molecular Biology Institute and the Departments of Anesthesiology and Medicine and ^||Molecular Pharmacology and Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 and the ^¶Department of Immunology, The Scripps Research Institute, La Jolla, California 92037

Stress-activated mitogen-activated protein (MAP) kinase p38 mediates stress signaling in mammalian cells via threonine and tyrosine phosphorylation in its conserved TGY motif by upstream MAP kinase kinases (MKKs). In addition, p38 MAP kinase can also be activated by an MKK-independent mechanism involving TAB-1 (TAK-1-binding protein)-mediated autophosphorylation. Although TAB-1-mediated p38 activation has been implicated in ischemic heart, the biological consequences and downstream signaling of TAB-1-mediated p38 activation in cardiomyocytes is largely unknown. We show here that TAB-1 expression leads to a significant induction of p38 autophosphorylation and consequent kinase activation in cultured neonatal cardiomyocytes. In contrast to MKK3-induced p38 kinase downstream effects, TAB-1-induced p38 kinase activation does not induce expression of pro-inflammatory genes, cardiac marker gene expression, or changes in cellular morphology. Rather, TAB-1 binds to p38 and prevents p38 nuclear localization. Furthermore, TAB-1 disrupts p38 interaction with MKK3 and redirects p38 localization in the cytosol. Consequently, TAB-1 expression antagonizes the downstream activity of p38 kinase induced by MKK3 and attenuates interleukin-1-induced inflammatory gene induction in cardiomyocytes. These data suggest that TAB-1 can mediate MKK-independent p38 kinase activation while negatively modulating MKK-dependent p38 function. Our study not only redefines the functional role of TAB-1 in p38 kinase-mediated signaling pathways but also provides the first evidence that intracellular localization of p38 kinase and complex interaction dictates its downstream effects. These results suggest a previously unknown mechanism for stress-MAP kinase regulation in mammalian cells.

Received for publication, July 13, 2005 , and in revised form, November 30, 2005.

^* This work was supported by funds from Division of Molecular Medicine, NIH Grants HL62311 and HL08111 (to Y. W.), NCI R01-CA84572 (to H. R. H.), and AI41637 and GM037696 (to J. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Division of Molecular Medicine, Depts. of Anesthesiology and Medicine, BH-569, CSH, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095. Tel.: 310-206-5197; E-mail: yibinwang{at}mednet.ucla.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				D. Westermann, T. Walther, K. Savvatis, F. Escher, M. Sobirey, A. Riad, M. Bader, H.-P. Schultheiss, and C. Tschope Gene Deletion of the Kinin Receptor B1 Attenuates Cardiac Inflammation and Fibrosis During the Development of Experimental Diabetic Cardiomyopathy Diabetes, June 1, 2009; 58(6): 1373 - 1381. [Abstract] [Full Text] [PDF]

				R. Lu, A. Alioua, Y. Kumar, P. Kundu, M. Eghbali, N. V. Weisstaub, J. A. Gingrich, E. Stefani, and L. Toro c-Src tyrosine kinase, a critical component for 5-HT2A receptor-mediated contraction in rat aorta J. Physiol., August 15, 2008; 586(16): 3855 - 3869. [Abstract] [Full Text] [PDF]

				A. Alioua, R. Lu, Y. Kumar, M. Eghbali, P. Kundu, L. Toro, and E. Stefani Slo1 Caveolin-binding Motif, a Mechanism of Caveolin-1-Slo1 Interaction Regulating Slo1 Surface Expression J. Biol. Chem., February 22, 2008; 283(8): 4808 - 4817. [Abstract] [Full Text] [PDF]

				J. S. Jaswal, M. Gandhi, B. A. Finegan, J. R. B. Dyck, and A. S. Clanachan p38 mitogen-activated protein kinase mediates adenosine-induced alterations in myocardial glucose utilization via 5'-AMP-activated protein kinase Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1978 - H1985. [Abstract] [Full Text] [PDF]

				C. Makino, Y. Sano, T. Shinagawa, J. B. A. Millar, and S. Ishii Sin1 binds to both ATF-2 and p38 and enhances ATF-2-dependent transcription in an SAPK signaling pathway Genes Cells, November 1, 2006; 11(11): 1239 - 1251. [Abstract] [Full Text] [PDF]

				B. Fiedler, R. Feil, F. Hofmann, C. Willenbockel, H. Drexler, A. Smolenski, S. M. Lohmann, and K. C. Wollert cGMP-dependent Protein Kinase Type I Inhibits TAB1-p38 Mitogen-activated Protein Kinase Apoptosis Signaling in Cardiac Myocytes J. Biol. Chem., October 27, 2006; 281(43): 32831 - 32840. [Abstract] [Full Text] [PDF]

				M. R. Morissette, S. A. Cook, S. Foo, G. McKoy, N. Ashida, M. Novikov, M. Scherrer-Crosbie, L. Li, T. Matsui, G. Brooks, et al. Myostatin Regulates Cardiomyocyte Growth Through Modulation of Akt Signaling Circ. Res., July 7, 2006; 99(1): 15 - 24. [Abstract] [Full Text] [PDF]