HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 1, 267-276, January 5, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/1/267    most recent M605463200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yao, D. Articles by Funk, C. Search for Related Content PubMed PubMed Citation Articles by Yao, D. Articles by Funk, C. Social Bookmarking                      What's this?

Localization of the Small CAB-like Proteins in Photosystem II^*

Danny Yao^¶, Thomas Kieselbach, Josef Komenda^||**, Kamoltip Promnares^||**, Miguel A. Hernández Prieto^¶, Martin Tichy^||**, Wim Vermaas^¶, and Christiane Funk¹

From the Department of Biochemistry and the Umeå Plant Science Centre, Umeå University, SE-901 87 Umeå, Sweden, the ^¶School of Life Sciences and the Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, Arizona 85287-4501, the ^||Institute of Physical Biology, University of South Bohemia, 373 33 Nové Hrady, Czech Republic, and the ^**Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences, 379 81 Trebon, Czech Republic

The cyanobacterial small CAB-like proteins (SCPs) consist of one-helix proteins that resemble transmembrane regions of the light-harvesting proteins of plants. To determine whether these proteins are associated with protein complexes in the thylakoid membrane, an abundant member of the SCP family, ScpD, was marked with a His tag, and proteins co-isolating with His-tagged ScpD were identified. These proteins included the major Photosystem (PS) II components as well as FtsH, which is involved in degradation of the PSII complex. To ascertain specific interaction between ScpD and the PSII complex, the His-tagged protein fraction was subjected to two-dimensional blue native/SDS-PAGE. Again, PSII components were co-isolated with ScpD-His, and ScpD-His was found to interact most strongly with CP47. ScpD association was most prominent with the monomeric form of PSII, suggesting ScpD association with PSII that is repaired. Using antibodies that recognize both ScpC and ScpD, we found the ScpC protein, which is very similar in primary structure to ScpD, to also co-isolate with the PSII complex. In contrast, ScpE did not co-isolate with a major protein complex in thylakoids. A fourth member of the SCP family, ScpB, could not be immunodetected, but was found by mass spectrometry in samples co-isolating with ScpD-His. Therefore, ScpB may be associated with ScpD as well. No association between SCPs and PSI could be demonstrated. On the basis of these and other data presented, we suggest that members of the SCP family can associate with damaged PSII and can serve as a temporary pigment reservoir while PSII components are being replaced.

Received for publication, June 7, 2006

^* The work was supported by the Swedish Foundation for International Cooperation in Research and Higher Education, the Swedish Research Council, Department of Energy Grant DE-FG02-04ER15543 (to W. R.), and Czech Academy of Sciences Institutional Research Concept AV0Z50200510 and Project MSM6007665808 (to J. K. and M. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 46-90-786-7633; Fax: 46-90-786-7661; E-mail: Christiane.Funk{at}chem.umu.se.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Dobakova, R. Sobotka, M. Tichy, and J. Komenda Psb28 Protein Is Involved in the Biogenesis of the Photosystem II Inner Antenna CP47 (PsbB) in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiology, February 1, 2009; 149(2): 1076 - 1086. [Abstract] [Full Text] [PDF]

				Q. Wang, S. Jantaro, B. Lu, W. Majeed, M. Bailey, and Q. He The High Light-Inducible Polypeptides Stabilize Trimeric Photosystem I Complex under High Light Conditions in Synechocystis PCC 6803 Plant Physiology, July 1, 2008; 147(3): 1239 - 1250. [Abstract] [Full Text] [PDF]

				D. Vavilin, D. Yao, and W. Vermaas Small Cab-like Proteins Retard Degradation of Photosystem II-associated Chlorophyll in Synechocystis sp. PCC 6803: KINETIC ANALYSIS OF PIGMENT LABELING WITH 15N AND 13C J. Biol. Chem., December 28, 2007; 282(52): 37660 - 37668. [Abstract] [Full Text] [PDF]