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J. Biol. Chem., Vol. 282, Issue 11, 7869-7876, March 16, 2007

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Phosphorylation of Adult Type Sept5 (CDCrel-1) by Cyclin-dependent Kinase 5 Inhibits Interaction with Syntaxin-1^*

Makoto Taniguchi¹, Masato Taoka, Makoto Itakura^¶, Akiko Asada, Taro Saito, Makoto Kinoshita^||, Masami Takahashi^¶, Toshiaki Isobe, and Shin-ichi Hisanaga²

From the Departments of Biological Sciences and Chemistry, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397, the ^¶Department of Biochemistry, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, and the ^||Biochemistry and Cell Biology Unit, HMRO, Kyoto University Graduate School of Medicine, Sakyo, Kyoto 606-8501, Japan

Increasing evidence implicates cyclin-dependent kinase 5 (Cdk5) in neuronal synaptic function. We searched for Cdk5 substrates in synaptosomal fractions prepared from mouse brains. Mass spectrometric analysis after two-dimensional SDS-PAGE identified several synaptic proteins phosphorylated by Cdk5-p35; one protein identified was Sept5 (CDCrel-1). Although septins were isolated originally as cell division-related proteins in yeast, Sept5 is expressed predominantly in neurons and is implicated in exocytosis. We confirmed that Sept5 is phosphorylated by Cdk5-p35 in vitro and identified Ser¹⁷ of adult type Sept5 (Sept5_v1) as a major phosphorylation site. We found that Ser¹⁷ of Sept5_v1 is phosphorylated in mouse brains. Coimmunoprecipitation from synaptosomal fractions and glutathione S-transferase-syntaxin-1A pulldown assays of Sept5_v1 expressed in COS-7 cells showed that phosphorylation of Sept5_v1 by Cdk5-p35 decreases the binding to syntaxin-1. These results indicate that the interaction of Sept5 with syntaxin-1 is regulated by the phosphorylation of Sept5_v1 at Ser¹⁷ by Cdk5-p35.

Received for publication, October 6, 2006 , and in revised form, December 28, 2006.

^* This work was supported in part by Grants-in-Aid for Scientific Research on Priority Area, Research on Pathomechanisms of Brain Disorders from MEXT of Japan (to S. H.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence may be addressed: Dept. of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachiohji, Tokyo 192-0397, Japan. Tel.: 81-426-77-2577; Fax: 81-426-77-2559; E-mail: taniguti-makoto{at}c.metro-u.ac.jp. ² To whom correspondence may be addressed: Dept. of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachiohji, Tokyo 192-0397, Japan. Tel.: 81-426-77-2577; Fax: 81-426-77-2559; E-mail: hisanaga-shinichi{at}c.metro-u.ac.jp.

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