HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 12, 8734-8740, March 23, 2007

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 282/12/8734    most recent M608477200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ball, J. R. Articles by Ullman, K. S. Search for Related Content PubMed PubMed Citation Articles by Ball, J. R. Articles by Ullman, K. S. Social Bookmarking                      What's this?

Sequence Preference in RNA Recognition by the Nucleoporin Nup153^*

From the Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112

The vertebrate nuclear pore protein Nup153 contains a novel RNA binding domain. This 150-amino acid region was previously found to bind preferentially to a panel of mRNAs when compared with structured RNAs, such as tRNA, U snRNA, and double-stranded RNA. The ability to broadly recognize mRNA led to the conclusion that the Nup153 RNA binding domain confers a general affinity for single-stranded RNA. Here, we have probed Nup153 RNA recognition to decipher how this unique RNA binding domain discriminates between potential targets. We first mapped the binding determinant within an RNA fragment that associates relatively robustly with the Nup153 RNA binding domain. We next designed synthetic RNA oligonucleotides to systematically delineate the features within this minimal RNA fragment that are key to Nup153 RNA-binding domain binding and demonstrated that the binding preferences of Nup153 do not reflect general preferences of an mRNA/single-stranded RNA-binding protein. We further found that the association between Nup153 and a cellular mRNA can be attributed to an interaction with specific subregions of the RNA. These results indicate that Nup153 can discriminate between mRNA and other classes of RNA transcripts due in part to direct recognition of a loose sequence motif. This information adds a new dimension to the interfaces that can contribute to recognition in mRNA export cargo selection and fate.

Received for publication, September 5, 2006 , and in revised form, January 18, 2007.

^* This work was supported by National Institutes of Health Grant GM61275 (to K. S. U.) and a Ruth L. Kirschstein Individual National Service Award (to C. D.). Core facilities used in this study were partially supported by National Institutes of Health Grant P30 CA421014. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Tables S1 and S2.

¹ These authors contributed equally to this work.

² To whom correspondence should be addressed: Dept. of Oncological Sciences, 2000 Circle of Hope, Huntsman Cancer Inst., University of Utah, Salt Lake City, UT 84112. Tel.: 801-585-7123; Fax: 801-585-0900; E-mail: katharine.ullman{at}hci.utah.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. L. Woodward, S. Prakobwanakit, S. Mosessian, and S. A. Chow Integrase Interacts with Nucleoporin NUP153 To Mediate the Nuclear Import of Human Immunodeficiency Virus Type 1 J. Virol., July 1, 2009; 83(13): 6522 - 6533. [Abstract] [Full Text] [PDF]