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J. Biol. Chem., Vol. 282, Issue 15, 10915-10921, April 13, 2007

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Late Assembly Steps and Dynamics of the Cyanobacterial Photosystem I^*

Ulf Dühring, Friedrich Ossenbühl, and Annegret Wilde¹

From the Institute of Biology, Humboldt University Berlin, 10115 Berlin, Germany and the Department of Molecular Botany, University Ulm, 89069 Ulm, Germany

The dynamics of photosystem I assembly in cyanobacteria have been addressed using in vivo pulse-chase labeling of Synechocystis sp. PCC 6803 proteins in combination with blue native polyacrylamide gel electrophoresis. The analyses indicate the existence of three different monomeric photosystem I complexes and also the high stability of photosystem I trimers. We show that in addition to a complete photosystem I monomer, containing all 11 subunits, we detected a PsaK-less monomer and a short-lived PsaL/PsaK-less complex. The latter two monomers were missing in the ycf37 mutant of Synechocystis sp. PCC 6803 that accumulates also less trimers. Pulse-chase experiments suggest that the three monomeric complexes have different functions in the biogenesis of the trimer. Based on these findings we propose a model where PsaK is incorporated in the latest step of photosystem I assembly. The PsaK-less photosystem I monomer may represent an intermediate complex that is important for the exchange of the two PsaK variants during high light acclimation. Implications of the presented data with respect to Ycf37 function are discussed.

Received for publication, September 28, 2006 , and in revised form, February 7, 2007.

^* This work was supported by the Deutsche Forschungsgemeinschaft (SFB429, TP A8) and Fonds der Chemischen Industrie. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Institut für Biologie/Biochemie der Pflanzen, Humboldt-Universität zu Berlin, Chausseestr. 117, 10115 Berlin, Germany. Tel.: 493020938167; Fax: 493020938164; E-mail: Annegret.Wilde{at}rz.hu-berlin.de.

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