HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 17, 12860-12870, April 27, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/17/12860    most recent M611593200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pratt, J. T. Articles by Camilli, A. Search for Related Content PubMed PubMed Citation Articles by Pratt, J. T. Articles by Camilli, A. Social Bookmarking                      What's this?

PilZ Domain Proteins Bind Cyclic Diguanylate and Regulate Diverse Processes in Vibrio cholerae^*

From the Howard Hughes Medical Institute and the Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111

Cyclic diguanylate (c-di-GMP) is an allosteric activator and second messenger implicated in the regulation of a variety of biological processes in diverse bacteria. In Vibrio cholerae, c-di-GMP has been shown to inversely regulate biofilm-specific and virulence gene expression, suggesting that c-di-GMP signaling is important for the transition of V. cholerae from the environment to the host. However, the mechanism behind this regulation remains unknown. Recently, it was proposed that the PilZ protein domain represents a c-di-GMP-binding domain. Here we show that V. cholerae PilZ proteins bind c-di-GMP specifically and are involved in the regulation of biofilm formation, motility, and virulence. These findings confirm a role for PilZ proteins as c-di-GMP-sensing proteins within the c-di-GMP signaling network.

Received for publication, December 19, 2006

^* This work was supported by National Institutes of Health Grant AI45746 (to A. C.) and Center for Gastroenterology Research on Absorptive and Secretory Processes, New England Medical Center, Grant P30DK34928. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement"in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ Present address: Laboratory of Infection Biology, The Rockefeller University, 1230 York Ave., New York, NY 10021.

² To whom correspondence should be addressed: Howard Hughes Medical Institute and Dept. of Molecular Biology and Microbiology, Tufts University, 136 Harrison Ave., Boston, MA 02111. Tel.: 617-636-2144; Fax: 617-636-2175; E-mail: andrew.camilli{at}tufts.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E. Karatan and P. Watnick Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms Microbiol. Mol. Biol. Rev., June 1, 2009; 73(2): 310 - 347. [Abstract] [Full Text] [PDF]

				C. Solano, B. Garcia, C. Latasa, A. Toledo-Arana, V. Zorraquino, J. Valle, J. Casals, E. Pedroso, and I. Lasa Genetic reductionist approach for dissecting individual roles of GGDEF proteins within the c-di-GMP signaling network in Salmonella PNAS, May 12, 2009; 106(19): 7997 - 8002. [Abstract] [Full Text] [PDF]

				P. D. Newell, R. D. Monds, and G. A. O'Toole LapD is a bis-(3',5')-cyclic dimeric GMP-binding protein that regulates surface attachment by Pseudomonas fluorescens Pf0-1 PNAS, March 3, 2009; 106(9): 3461 - 3466. [Abstract] [Full Text] [PDF]

				S. Beyhan, L. S. Odell, and F. H. Yildiz Identification and Characterization of Cyclic Diguanylate Signaling Systems Controlling Rugosity in Vibrio cholerae J. Bacteriol., November 15, 2008; 190(22): 7392 - 7405. [Abstract] [Full Text] [PDF]

				M. R. Neunuebel and J. W. Golden The Anabaena sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions J. Bacteriol., October 15, 2008; 190(20): 6829 - 6836. [Abstract] [Full Text] [PDF]

				H. F. Martinez-Wilson, R. Tamayo, A. D. Tischler, D. W. Lazinski, and A. Camilli The Vibrio cholerae Hybrid Sensor Kinase VieS Contributes to Motility and Biofilm Regulation by Altering the Cyclic Diguanylate Level J. Bacteriol., October 1, 2008; 190(19): 6439 - 6447. [Abstract] [Full Text] [PDF]

				L. M. Holland, S. T. O'Donnell, D. A. Ryjenkov, L. Gomelsky, S. R. Slater, P. D. Fey, M. Gomelsky, and J. P. O'Gara A Staphylococcal GGDEF Domain Protein Regulates Biofilm Formation Independently of Cyclic Dimeric GMP J. Bacteriol., August 1, 2008; 190(15): 5178 - 5189. [Abstract] [Full Text] [PDF]

				A. Nakhamchik, C. Wilde, and D. A. Rowe-Magnus Cyclic-di-GMP Regulates Extracellular Polysaccharide Production, Biofilm Formation, and Rugose Colony Development by Vibrio vulnificus Appl. Envir. Microbiol., July 1, 2008; 74(13): 4199 - 4209. [Abstract] [Full Text] [PDF]

				R. Tamayo, S. Schild, J. T. Pratt, and A. Camilli Role of Cyclic Di-GMP during El Tor Biotype Vibrio cholerae Infection: Characterization of the In Vivo-Induced Cyclic Di-GMP Phosphodiesterase CdpA Infect. Immun., April 1, 2008; 76(4): 1617 - 1627. [Abstract] [Full Text] [PDF]

				A. J. Wolfe and K. L. Visick Get the Message Out: Cyclic-Di-GMP Regulates Multiple Levels of Flagellum-Based Motility J. Bacteriol., January 15, 2008; 190(2): 463 - 475. [Full Text] [PDF]

				L. Gualdi, L. Tagliabue, and P. Landini Biofilm Formation-Gene Expression Relay System in Escherichia coli: Modulation of {sigma}S-Dependent Gene Expression by the CsgD Regulatory Protein via {sigma}S Protein Stabilization J. Bacteriol., November 15, 2007; 189(22): 8034 - 8043. [Abstract] [Full Text] [PDF]