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J. Biol. Chem., Vol. 282, Issue 20, 14853-14860, May 18, 2007

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The LG1-3 Tandem of Laminin 5 Harbors the Binding Sites of Lutheran/Basal Cell Adhesion Molecule and 31/61 Integrins^*

Yamato Kikkawa^¶1, Takako Sasaki^||, Mai Tuyet Nguyen^||, Motoyoshi Nomizu^¶, Toshihiro Mitaka, and Jeffrey H. Miner^**

From the Renal Division, Department of Internal Medicine and ^**Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan, ^¶Laboratory of Clinical Biochemistry, Tokyo University of Pharmacy and Life Science, Tokyo 192-0392, Japan, and ^||Max-Planck-Institut fur Biochemie, D-82152 Martinsried, Germany

The laminin-type globular (LG) domains of laminin chains have been implicated in various cellular interactions that are mediated through receptors such as integrins, -dystroglycan, syndecans, and the Lutheran blood group glycoprotein (Lu). Lu, an Ig superfamily transmembrane receptor specific for laminin 5, is also known as basal cell adhesion molecule (B-CAM). Although Lu/B-CAM binds to the LG domain of laminin 5, the binding site has not been precisely defined. To better delineate this binding site, we produced a series of recombinant laminin trimers containing modified chains, such that all or part of 5LG was replaced with analogous segments of human laminin 1LG. In solid phase binding assays using a soluble Lu (Lu-Fc) composed of the Lu extracellular domain and human IgG1 Fc, we found that Lu bound to Mr5G3, a recombinant laminin containing 5 domains LN through LG3 fused to human laminin 1LG4–5. However, Lu/B-CAM did not bind other recombinant laminins containing 5LG3 unless 5LG1–2 was also present. A recombinant 5LG1-3 tandem lacking the laminin coiled coil (LCC) domain did not reproduce the activity of Lu/B-CAM binding. Therefore, proper structure of the 5LG1-3 tandem with the LCC domain was essential for the binding of Lu/B-CAM to laminin 5. Our results also suggest that the binding site for Lu/B-CAM on laminin 5 may overlap with that of integrins 31 and 61.

Received for publication, December 21, 2006 , and in revised form, March 23, 2007.

^* This work was supported, in part, by Grants 17590307 and 18060041 from the Ministry of Education, Sciences, Sports, and Culture, Japan (to Y. K.) and National Institutes of Health Grants R01DK064687 and R01GM060432 (to J. H. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains a supplemental figure.

¹ To whom correspondence should be addressed: Tokyo University of Pharmacy and Life Science, School of Pharmacy, Laboratory of Clinical Biochemistry, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Tel.: 81-42-676-5670; Fax: 81-42-676-5669; E-mail: kikkawa{at}ps.toyaku.ac.jp.

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