HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 21, 15768-15777, May 25, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/21/15768    most recent M700397200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kim, D. Articles by Tucker, P. W. Search for Related Content PubMed PubMed Citation Articles by Kim, D. Articles by Tucker, P. W. Social Bookmarking                      What's this?

REKLES Is an ARID3-restricted Multifunctional Domain^*

From the Section of Molecular Genetics and Microbiology and Institute of Cell and Molecular Biology, University of Texas, Austin, Texas 78712-0162

Bright/Dril1/ARID3a is a B cell-specific, matrix association (or attachment) region-binding transcriptional regulator of immunoglobulin heavy chain genes and of E2F1-dependent cell cycle progression. Bright contains a central DNA binding domain termed ARID (AT-rich interacting domain) and a C-terminal region termed REKLES (for a conserved amino acid motif). The ARID domain has been identified in seven highly conserved families of metazoan proteins (ARID1-5 and JARID1-2), whereas REKLES is found only in the ARID3 subfamily (composed of Bright/ARID3a, Bdp/ARID3b, and Bright-like/ARID3c). REKLES consists of two subdomains: a modestly conserved N-terminal REKLES and a highly conserved (among ARID3 orthologous proteins) C-terminal REKLES. Previously we showed that Bright undergoes nucleocytoplasmic shuttling and that REKLES and - were required, respectively, for nuclear import and Crm1-dependent nuclear export. Here we show that Bright further requires REKLES for self-association or paralogue association and for nuclear matrix targeting. REK-LES promotes and regulates the extent of Bright multimerization, which occurs in the absence or presence of target DNA and is necessary for specific DNA binding. REKLES-mediated interaction of Bright with Bdp, which localizes strictly to the nucleus, traps Bright within the nucleus via neutralization of its nuclear export activity. These results identify REKLES as a multifunctional domain that has co-evolved with and regulates functional properties of the ARID3 DNA binding domain.

Received for publication, January 16, 2007 , and in revised form, March 23, 2007.

^* This work was supported by the National Institutes of Health and the Mary Betzner Morrow Endowment. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Molecular Genetics and Microbiology, the University of Texas, 1 University Station A5000, NMS1.124, Austin, TX 78712-0162. Tel.: 512-475-7705; Fax: 512-475-7707; E-mail: philtucker{at}mail.utexas.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?