HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 23, 16736-16743, June 8, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/23/16736    most recent M611345200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nebauer, R. Articles by Daum, G. Search for Related Content PubMed PubMed Citation Articles by Nebauer, R. Articles by Daum, G. Social Bookmarking                      What's this?

Phosphatidylethanolamine, a Limiting Factor of Autophagy in Yeast Strains Bearing a Defect in the Carboxypeptidase Y Pathway of Vacuolar Targeting^*

From the Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, A-8010 Graz, Austria

Vps4p and Vps36p of Saccharomyces cerevisiae are involved in the transport of proteins to the vacuole via the carboxypeptidase Y pathway. We found that deletion of VPS4 and VPS36 caused impaired maturation of the vacuolar proaminopeptidase I (pAPI) via autophagy or the cytosol to vacuole targeting pathway. Supplementation with ethanolamine rescued this defect, leading to an increase of the cellular amount of phosphatidylethanolamine (PtdEtn), an enhanced level of the PtdEtn-binding autophagy protein Atg8p and a balanced rate of autophagy. We also discovered that maturation of pAPI was generally affected by PtdEtn depletion in a psd1 psd2 mutant due to reduced recruitment of Atg8p to the preautophagosomal structure. Ethanolamine supplementation provided the necessary amounts of PtdEtn for complete maturation of pAPI. Since the expression level of Atg8p was not compromised in the psd1 psd2 strain, we concluded that the amount of available PtdEtn was limiting. Thus, PtdEtn appears to be a limiting factor for the balance of the carboxypeptidase Y pathway and autophagy/the cytosol to vacuole targeting pathway in the yeast.

Received for publication, December 11, 2006 , and in revised form, March 30, 2007.

^* This work was supported by the Fonds zur Förderung der Wissenschaftlichen Forschung in Österreich Project 17321 (to G. D.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Institute of Biochemistry, Graz University of Technology, Petersgasse 12/2, A-8010 Graz, Austria. Tel.: 43-316-873-6462; Fax: 43-316-873-6952; E-mail: guenther.daum{at}tugraz.at.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. Luo, Y. Matsuo, G. Gulis, H. Hinz, J. Patton-Vogt, and S. Marcus Phosphatidylethanolamine Is Required for Normal Cell Morphology and Cytokinesis in the Fission Yeast Schizosaccharomyces pombe Eukaryot. Cell, May 1, 2009; 8(5): 790 - 799. [Abstract] [Full Text] [PDF]

				L. A. Schroder, M. V. Ortiz, and W. A. Dunn Jr. The Membrane Dynamics of Pexophagy Are Influenced by Sar1p in Pichia pastoris Mol. Biol. Cell, November 1, 2008; 19(11): 4888 - 4899. [Abstract] [Full Text] [PDF]

				J. E. Vance Thematic Review Series: Glycerolipids. Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids J. Lipid Res., July 1, 2008; 49(7): 1377 - 1387. [Abstract] [Full Text] [PDF]