HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 25, 18481-18496, June 22, 2007

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 282/25/18481    most recent M611330200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Minami, Y. Articles by Takai, Y. Search for Related Content PubMed PubMed Citation Articles by Minami, Y. Articles by Takai, Y. Social Bookmarking                      What's this?

Necl-5/Poliovirus Receptor Interacts in cis with Integrin _V3 and Regulates Its Clustering and Focal Complex Formation^*

Yukiko Minami, Wataru Ikeda, Mihoko Kajita, Tsutomu Fujito, Hisayuki Amano, Yoshiyuki Tamaru, Kaori Kuramitsu, Yasuhisa Sakamoto, Morito Monden, and Yoshimi Takai¹

From the Departments of Molecular Biology and Biochemistry and Surgery and Clinical Oncology, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Osaka, Japan

Integrin _v3, which forms focal complexes at leading edges in moving cells, is up-regulated in cancer cells and so is implicated in their invasiveness. Necl-5, originally identified as a poliovirus receptor and also up-regulated in cancer cells, colocalizes with integrin _v3 at leading edges in moving cells and enhances growth factor-induced cell movement. Here, we show that Necl-5 interacts directly, in cis, with integrin _v3, and enhances integrin _v3 clustering and focal complex formation at leading edges in NIH3T3 cells. The extracellular region of Necl-5, but not the cytoplasmic region, is necessary for its interaction with integrin _v3; however, both regions are necessary for its action. An interaction between integrin _v3 and vitronectin and PDGF-induced activation of Rac are also necessary for integrin _v3 clustering. The interaction between Necl-5 and integrin _v3 enhances PDGF-induced Rac activation, facilitating integrin _v3 clustering presumably in a feedback amplification manner. Thus, Necl-5 has a critical role in integrin _v3 clustering and focal complex formation.

Received for publication, December 11, 2006 , and in revised form, March 20, 2007.

^* This work was supported by grants-in-aid for Scientific Research and for Cancer Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (2005, 2006). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. S1-S3.

¹ To whom correspondence should be addressed: Dept. of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita, Osaka 565-0871, Japan. Tel.: 81-6-6879-3410; Fax: 81-6-6879-3419; E-mail: ytakai{at}molbio.med.osaka-u.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. Kanzaki, H. Ogita, H. Komura, M. Ozaki, Y. Sakamoto, T. Majima, T. Ijuin, T. Takenawa, and Y. Takai Involvement of the nectin-afadin complex in PDGF-induced cell survival J. Cell Sci., June 15, 2008; 121(12): 2008 - 2017. [Abstract] [Full Text] [PDF]

				M. Takahashi, Y. Rikitake, Y. Nagamatsu, T. Hara, W. Ikeda, K.-i. Hirata, and Y. Takai Sequential activation of Rap1 and Rac1 small G proteins by PDGF locally at leading edges of NIH3T3 cells. Genes Cells, June 1, 2008; 13(6): 549 - 569. [Abstract] [Full Text] [PDF]

				Y. Nagamatsu, Y. Rikitake, M. Takahashi, Y. Deki, W. Ikeda, K.-i. Hirata, and Y. Takai Roles of Necl-5/Poliovirus Receptor and Rho-associated Kinase (ROCK) in the Regulation of Transformation of Integrin {alpha}V{beta}3-based Focal Complexes into Focal Adhesions J. Biol. Chem., May 23, 2008; 283(21): 14532 - 14541. [Abstract] [Full Text] [PDF]

				H. Amano, W. Ikeda, S. Kawano, M. Kajita, Y. Tamaru, N. Inoue, Y. Minami, A. Yamada, and Y. Takai Interaction and localization of Necl-5 and PDGF receptor beta at the leading edges of moving NIH3T3 cells: Implications for directional cell movement. Genes Cells, March 1, 2008; 13(3): 269 - 284. [Abstract] [Full Text] [PDF]

				Y. Sakamoto, H. Ogita, H. Komura, and Y. Takai Involvement of Nectin in Inactivation of Integrin {alpha}v 3 after the Establishment of Cell-Cell Adhesion J. Biol. Chem., January 4, 2008; 283(1): 496 - 505. [Abstract] [Full Text] [PDF]

				S. Nakata, N. Fujita, Y. Kitagawa, R. Okamoto, H. Ogita, and Y. Takai Regulation of Platelet-derived Growth Factor Receptor Activation by Afadin through SHP-2: IMPLICATIONS FOR CELLULAR MORPHOLOGY J. Biol. Chem., December 28, 2007; 282(52): 37815 - 37825. [Abstract] [Full Text] [PDF]