HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 25, 18613-18624, June 22, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/25/18613    most recent M701983200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Viswakarma, N. Articles by Reddy, J. K. Search for Related Content PubMed PubMed Citation Articles by Viswakarma, N. Articles by Reddy, J. K. Social Bookmarking                      What's this?

Transcriptional Regulation of Cidea, Mitochondrial Cell Death-inducing DNA Fragmentation Factor -Like Effector A, in Mouse Liver by Peroxisome Proliferator-activated Receptor and ^*

From the Department of Pathology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611-3008

Cidea (cell death-inducing DNA fragmentation factor -like effector A), a member of a novel family of proapoptotic proteins, is expressed abundantly in the brown adipose tissue of the mouse. Although Cidea mRNA is not detectable in the mouse liver, we now show that peroxisome proliferator-activated receptor (PPAR) ligands Wy-14,643 and ciprofibrate increase the Cidea mRNA level in a PPAR-dependent manner, whereas Cidea induction in liver by PPAR overexpression is PPAR independent. Increase in Cidea mRNA content in liver did not alter the expression of uncoupling protein 1 (Ucp1) gene, which regulates thermogenesis, lipolysis, and conservation of energy. Although Cidea is considered to be a proapoptotic factor, Cidea induction in liver did not result in increased apoptosis. To elucidate the mechanism by which PPAR and PPAR regulate Cidea gene expression in the liver, we analyzed the promoter region of the Cidea gene. Three putative peroxisome proliferator response elements (PPREs) are found in the Cidea gene promoter. Transactivation, gel-shift, and chromatin immunoprecipitation assays indicated that the proximal PPRE in Cidea gene (Cidea-PPRE1 at -680/-668) is functional for both PPAR and -. We conclude that Cidea is a novel target gene for both PPAR and - in the liver where these two transcription factors utilize the same PPRE region for dual regulation. The induction of Cidea in liver with these PPAR and - agonists suggests a possible role for Cidea in energy metabolism and a less likely role in hepatocyte apoptosis.

Received for publication, March 7, 2007 , and in revised form, April 26, 2007.

^* This work was supported in part by National Institutes of Health Grants GM23750 (to J. K. R.) and CA104578 (to J. K. R.) and by the Mayberry Endowment Fund. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 312-503-7948; E-mail: jkreddy{at}northwestern.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Hallberg, D. L. Morganstein, E. Kiskinis, K. Shah, A. Kralli, S. M. Dilworth, R. White, M. G. Parker, and M. Christian A Functional Interaction between RIP140 and PGC-1{alpha} Regulates the Expression of the Lipid Droplet Protein CIDEA Mol. Cell. Biol., November 15, 2008; 28(22): 6785 - 6795. [Abstract] [Full Text] [PDF]

				V. Puri, S. Ranjit, S. Konda, S. M. C. Nicoloro, J. Straubhaar, A. Chawla, M. Chouinard, C. Lin, A. Burkart, S. Corvera, et al. Cidea is associated with lipid droplets and insulin sensitivity in humans PNAS, June 3, 2008; 105(22): 7833 - 7838. [Abstract] [Full Text] [PDF]

				J. Y. Kim, K. Liu, S. Zhou, K. Tillison, Y. Wu, and C. M. Smas Assessment of fat-specific protein 27 in the adipocyte lineage suggests a dual role for FSP27 in adipocyte metabolism and cell death Am J Physiol Endocrinol Metab, April 1, 2008; 294(4): E654 - E667. [Abstract] [Full Text] [PDF]

				D. Li, L. Da, H. Tang, T. Li, and M. Zhao CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding Nucleic Acids Res., January 17, 2008; 36(1): 330 - 341. [Abstract] [Full Text] [PDF]