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J. Biol. Chem., Vol. 282, Issue 28, 20388-20394, July 13, 2007

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Fission Yeast Rnf4 Homologs Are Required for DNA Repair^*

From the Department of Oncological Sciences, Mount Sinai School of Medicine, New York, New York 10029

We describe two RING finger proteins in the fission yeast Schizosaccharomyces pombe, Rfp1 and Rfp2. We show that these proteins function redundantly in DNA repair. Rfp1 was isolated as a Chk1-interacting protein in a two-hybrid screen and has high amino acid sequence similarity to Rfp2. Deletion of either gene does not cause a phenotype, but a double deletion (rfp1rfp2) showed poor viability and defects in cell cycle progression. These cells are also sensitive to DNA-damaging agents, although they maintained normal checkpoint signaling to Chk1. Rfp1 and Rfp2 are most closely related to human Rnf4, and we showed that Rnf4 can substitute functionally for Rfp1 and/or Rfp2. The double mutants also showed significantly increased levels of protein SUMOylation, and we identified an S. pombe Ulp2/Smt4 homolog that, when overexpressed, reduced SUMO levels and suppressed the DNA damage sensitivity of rfp1 rfp2 cells.

Received for publication, March 28, 2007 , and in revised form, May 14, 2007.

^* This work was supported by National Institutes of Health/NCI Grant CA100076. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Oncological Sciences, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029. Tel.: 212-659-5468; Fax: 212-987-2240; E-mail: matthew.oconnell{at}mssm.edu.

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