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J. Biol. Chem., Vol. 282, Issue 30, 21639-21644, July 27, 2007

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Kinetic Characterization of Tail Swing Steps in the ATPase Cycle of Dictyostelium Cytoplasmic Dynein^*

Toshifumi Mogami, Takahide Kon, Kohji Ito, and Kazuo Sutoh¹

From the Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan and Department of Biology, Chiba University, Inage-ku, Chiba 263-8522, Japan

According to the power stroke model of dynein deduced from electron microscopic and fluorescence resonance energy transfer studies, the power stroke and the recovery stroke are expected to take place at the two isomerization steps of the ATPase cycle at the primary ATPase site. Here, we have conducted presteady-state kinetic analyses of these two isomerization steps with the single-headed motor domain of Dictyostelium cytoplasmic dynein by employing fluorescence resonance energy transfer to probe ATPase steps at the primary site and tail positions. Our results show that the recovery stroke at the first isomerization step proceeds quickly (180 s^–1), whereas the power stroke at the second isomerization step is very slow (0.2 s^–1) in the absence of microtubules, and that the presence of microtubules accelerates the second but not the first step. Moreover, a comparison of the microtubule-induced acceleration of the power stroke step and that of steady-state ATP hydrolysis implies the intriguing possibility that microtubules simultaneously accelerate the ATPase activity not only at the primary site but also at other site(s) in the motor domain.

Received for publication, March 5, 2007 , and in revised form, May 31, 2007.

^* This work was supported by a grant-in-aid for Scientific Research (S) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), a grant-in-aid for Scientific Research on Priority Areas from MEXT (to K. S.), a grant-in-aid for Young Scientists (B) from MEXT (to T. K.), by the Core Research for Evolutional Science and Technology program grant from the Japan Science and Technology Agency (to K. S.), and by a grant in aid for Japan Society for the Promotion of Science fellows (to T. M.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel./Fax: 81-3-5454-6751; E-mail: sutoh{at}bio.c.u-tokyo.ac.jp.

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This article has been cited by other articles:

				K. Imamula, T. Kon, R. Ohkura, and K. Sutoh The coordination of cyclic microtubule association/dissociation and tail swing of cytoplasmic dynein PNAS, October 9, 2007; 104(41): 16134 - 16139. [Abstract] [Full Text] [PDF]