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J. Biol. Chem., Vol. 282, Issue 34, 24752-24758, August 24, 2007

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Ligand-induced Degradation of the Ethylene Receptor ETR2 through a Proteasome-dependent Pathway in Arabidopsis^*

Yi-Feng Chen, Samina N. Shakeel, Julie Bowers, Xue-Chu Zhao, Naomi Etheridge, and G. Eric Schaller¹

From the Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755 and the Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, New Hampshire 03824

Protein degradation plays an important role in modulating ethylene signal transduction in plants. Here we show that the ethylene receptor ETR2 is one such target for degradation and that its degradation is dependent upon perception of the signaling ligand ethylene. The ETR2 protein is initially induced by ethylene treatment, consistent with an increase in transcript levels. At ethylene concentrations above 1 µl/liter, however, ETR2 protein levels subsequently decrease in a post-transcriptional fashion. Genetic and chemical approaches indicate that ethylene perception by the receptors initiates the reduction in ETR2 protein levels. The ethylene-induced decrease in ETR2 levels is not affected by cycloheximide, an inhibitor of protein biosynthesis, but is affected by proteasome inhibitors, indicating a role for the proteasome in ETR2 degradation. Ethylene-induced degradation still occurs in seedlings treated with brefeldin A, indicating that degradation of ETR2 does not require exit from its subcellular location at the endoplasmic reticulum. These data support a model in which ETR2 is degraded by a proteasome-dependent pathway in response to ethylene binding. Implications of this model for ethylene signaling are discussed.

Received for publication, May 30, 2007 , and in revised form, June 25, 2007.

^* This work was supported by Department of Energy Grant DE-FG02-05ER15704 and National Science Foundation Grant MCB-0430191 (to G. E. S.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 603-646-2525; Fax: 603-646-1347; E-mail: george.e.schaller{at}dartmouth.edu.

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