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J. Biol. Chem., Vol. 282, Issue 37, 26740-26745, September 14, 2007

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Fibromodulin Binds Collagen Type I via Glu-353 and Lys-355 in Leucine-rich Repeat 11^*

From the Department of Experimental Medical Science, University of Lund, SE-221 84 Lund, Sweden

Fibromodulin belongs to the small leucine-rich repeat proteoglycan family, interacts with collagen type I, and controls collagen fibrillogenesis and assembly. Here, we show that a major fibromodulin-binding site for collagen type I is located in leucine-rich repeat 11 in the C terminus of the leucine-rich repeat domain. We identified Glu-353 and Lys-355 in repeat 11 as essential for binding, and the synthetic peptide RLDGNEIKR, including Glu-353 and Lys-355, inhibits the binding of fibromodulin to collagen in vitro. Fibromodulin and lumican compete for the same binding region on collagen, and fibromodulin can inhibit the binding of lumican to collagen type I. However, the peptide RLDGNEIKR does not inhibit the binding of lumican to collagen, suggesting separate but closely situated fibromodulin- and lumican-binding sites in collagen. The collagen-binding Glu-353 and Lys-355 residues in fibromodulin are exposed on the exterior of the -sheet-loop structure of the leucine-rich repeat, which resembles the location of interacting residues in other leucine-rich repeat proteins, e.g. decorin.

Received for publication, May 16, 2007 , and in revised form, July 9, 2007.

^* This study was supported by grants from the Swedish Research Council, Gustaf V:s 80-Anniversary Fund, Greta and Johan Kock's Fund, and Alfred Österlund's Fund. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains Supplemental Fig. 1.

¹ To whom correspondence should be addressed: Lund University, Dept. of Experimental Medical Science, BMC B12, 221 84 Lund, Sweden. Tel.: 46-462228577; Fax: 46-462220855; E-mail: Ake.Oldberg{at}med.lu.se.

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