HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 39, 28929-28938, September 28, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/39/28929    most recent M702633200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, X.-M. Articles by LaRusso, N. F. Search for Related Content PubMed PubMed Citation Articles by Chen, X.-M. Articles by LaRusso, N. F. Social Bookmarking                      What's this?

A Cellular Micro-RNA, let-7i, Regulates Toll-like Receptor 4 Expression and Contributes to Cholangiocyte Immune Responses against Cryptosporidium parvum Infection^*

From the Miles and Shirley Fiterman Center for Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905

Toll-like receptors (TLRs) are important pathogen recognition molecules and are key to epithelial immune responses to microbial infection. However, the molecular mechanisms that regulate TLR expression in epithelia are obscure. Micro-RNAs play important roles in a wide range of biological events through post-transcriptional suppression of target mRNAs. Here we report that human biliary epithelial cells (cholangiocytes) express let-7 family members, micro-RNAs with complementarity to TLR4 mRNA. We found that let-7 regulates TLR4 expression via post-transcriptional suppression in cultured human cholangiocytes. Infection of cultured human cholangiocytes with Cryptosporidium parvum, a parasite that causes intestinal and biliary disease, results in decreased expression of primary let-7i and mature let-7 in a MyD88/NF-B-dependent manner. The decreased let-7 expression is associated with C. parvum-induced up-regulation of TLR4 in infected cells. Moreover, experimentally induced suppression or forced expression of let-7i causes reciprocal alterations in C. parvum-induced TLR4 protein expression, and consequently, infection dynamics of C. parvum in vitro. These results indicate that let-7i regulates TLR4 expression in cholangiocytes and contributes to epithelial immune responses against C. parvum infection. Furthermore, the data raise the possibility that micro-RNA-mediated post-transcriptional pathways may be critical to host-cell regulatory responses to microbial infection in general.

Received for publication, March 27, 2007 , and in revised form, July 26, 2007.

^* This work was supported by National Institutes of Health Grants AI071321 (to X.-M. C.), DK57993 and DK24031 (to N. F. L.) and the Mayo Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ These authors contributed equally to this work.

² To whom correspondence may be addressed: Dept. of Medical Microbiology and Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178. Tel.: 402-280-3750; Fax: 402-280-1875; E-mail: xianmingchen{at}creighton.edu.

³ To whom correspondence may be addressed: Mayo Clinic College of Medicine, 200 First St., SW, Rochester, MN 55905. Tel.: 507-284-1006; Fax: 507-284-0762; E-mail: larusso.nicholas{at}mayo.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. D. Kamda and S. M. Singer Phosphoinositide 3-Kinase-Dependent Inhibition of Dendritic Cell Interleukin-12 Production by Giardia lamblia Infect. Immun., February 1, 2009; 77(2): 685 - 693. [Abstract] [Full Text] [PDF]

				A.-Y. Gong, R. Zhou, G. Hu, X. Li, P. L. Splinter, S. P. O'Hara, N. F. LaRusso, G. A. Soukup, H. Dong, and X.-M. Chen MicroRNA-513 Regulates B7-H1 Translation and Is Involved in IFN-{gamma}-Induced B7-H1 Expression in Cholangiocytes J. Immunol., February 1, 2009; 182(3): 1325 - 1333. [Abstract] [Full Text] [PDF]

				N. Yang, S. Kaur, S. Volinia, J. Greshock, H. Lassus, K. Hasegawa, S. Liang, A. Leminen, S. Deng, L. Smith, et al. MicroRNA Microarray Identifies Let-7i as a Novel Biomarker and Therapeutic Target in Human Epithelial Ovarian Cancer Cancer Res., December 15, 2008; 68(24): 10307 - 10314. [Abstract] [Full Text] [PDF]

				R. Dai, R. A. Phillips, Y. Zhang, D. Khan, O. Crasta, and S. A. Ahmed Suppression of LPS-induced Interferon-{gamma} and nitric oxide in splenic lymphocytes by select estrogen-regulated microRNAs: a novel mechanism of immune modulation Blood, December 1, 2008; 112(12): 4591 - 4597. [Abstract] [Full Text] [PDF]

				F J Sheedy and L A J O'Neill Adding fuel to fire: microRNAs as a new class of mediators of inflammation Ann Rheum Dis, December 1, 2008; 67(Suppl_3): iii50 - iii55. [Abstract] [Full Text] [PDF]

				Y. Xu, X. Zhou, and W. Zhang MicroRNA prediction with a novel ranking algorithm based on random walks Bioinformatics, July 1, 2008; 24(13): i50 - i58. [Abstract] [Full Text] [PDF]

				U. Lakshmipathy and R. P. Hart Concise Review: MicroRNA Expression in Multipotent Mesenchymal Stromal Cells Stem Cells, February 1, 2008; 26(2): 356 - 363. [Abstract] [Full Text] [PDF]