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J. Biol. Chem., Vol. 282, Issue 43, 31341-31348, October 26, 2007

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Dynamic Processing of Recombinant Dentin Sialoprotein-Phosphophoryn Protein^*

From the Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078

Dentin sialoprotein (DSP) and phosphophoryn (PP) are the two noncollagenous proteins classically linked to dentin but more recently found in bone, kidney, and salivary glands. These two proteins are derived from a single copy DSP-PP gene. Although this suggests that the DSP-PP gene is first transcribed into DSP-PP mRNAs, which later undergo processing to yield the DSP and PP proteins, this mechanism has not yet been demonstrated because of the inability to identify a DSP-PP precursor protein from any cell or tissue sample. To study this problem, we utilized a baculovirus expression system to produce recombinant DSP-PP precursor proteins from a DSP-PP₂₄₀ cDNA, which represents one of several endogenous DSP-PP transcripts that influence various tooth mineralization phases. Our in vitro results demonstrate that DSP-PP₂₄₀ precursor proteins are produced by this system and are capable of self-processing to yield both DSP and PP proteins. We further demonstrated that purified recombinant DSP-PP₂₄₀, purified recombinant PP₂₄₀, and the native highly phosphorylated protein (equivalent to the PP₅₂₃ isoform) have proteolytic activity. These newly identified tissue proteases may play key roles in tissue modeling during organogenesis.

Received for publication, March 27, 2007 , and in revised form, July 27, 2007.

^* This work was supported by National Insitutes of Health Grant DE11442 (to H. H. R.). The Michigan Proteome Consortium is supported in part by funds from the Michigan Life Sciences Corridor (State of Michigan MEDC Grant GR239). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Rm. 2393, Dental Bldg., University of Michigan School of Dentistry, 1011 N. University Ave., Ann Arbor, MI 48109-1078. Tel.: 734-763-3746; Fax: 734-936-1597; E-mail: helenar{at}umich.edu.

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