HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 47, 33897-33901, November 23, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/47/33897    most recent C700181200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sun, Y.-H. Articles by Tsolis, R. M. Search for Related Content PubMed PubMed Citation Articles by Sun, Y.-H. Articles by Tsolis, R. M. Social Bookmarking                      What's this?

Injection of Flagellin into the Host Cell Cytosol by Salmonella enterica Serotype Typhimurium^*

From the Department of Medical Microbiology and Immunology, University of California at Davis, Davis, California, 95616

Bacterial flagellins are potent inducers of innate immunity. Three signaling pathways have been implicated in the sensing of flagellins; these involve toll-like receptor 5 (TLR5) and the cytosolic proteins Birc1e/Naip5 and Ipaf. Although the structural basis of TLR5-flagellin interaction is known, little is known about how flagellin enters the host cell cytosol to induce signaling via Birc1e/Naip5 and Ipaf. Here we demonstrate for the first time the translocation of bacterial flagellin into the cytosol of host macrophages by the vacuolar pathogen, Salmonella enterica serotype Typhimurium. Translocation of flagellin into the host cell cytosol was directly demonstrated using -lactamase reporter constructs. Flagellin translocation required the Salmonella Pathogenicity Island 1 Type III secretion system (SPI-1 T3SS) but not the flagellar T3SS.

Received for publication, September 13, 2007

^* This work was supported by U.S. Public Health Service Grants AI50553 and AI065739. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Medical Microbiology and Immunology, University of California at Davis, One Shields Ave., Davis, CA 95616. Fax: 530-754-7240; E-mail: rmtsolis{at}ucdavis.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. E. Winter, P. Thiennimitr, S.-P. Nuccio, T. Haneda, M. G. Winter, R. P. Wilson, J. M. Russell, T. Henry, Q. T. Tran, S. D. Lawhon, et al. Contribution of Flagellin Pattern Recognition to Intestinal Inflammation during Salmonella enterica Serotype Typhimurium Infection Infect. Immun., May 1, 2009; 77(5): 1904 - 1916. [Abstract] [Full Text] [PDF]

				D. Prantner and U. M. Nagarajan Role for the Chlamydial Type III Secretion Apparatus in Host Cytokine Expression Infect. Immun., January 1, 2009; 77(1): 76 - 84. [Abstract] [Full Text] [PDF]

				I. Godinez, M. Raffatellu, H. Chu, T. A. Paixao, T. Haneda, R. L. Santos, C. L. Bevins, R. M. Tsolis, and A. J. Baumler Interleukin-23 Orchestrates Mucosal Responses to Salmonella enterica Serotype Typhimurium in the Intestine Infect. Immun., January 1, 2009; 77(1): 387 - 398. [Abstract] [Full Text] [PDF]

				S. E. Warren, D. P. Mao, A. E. Rodriguez, E. A. Miao, and A. Aderem Multiple Nod-Like Receptors Activate Caspase 1 during Listeria monocytogenes Infection J. Immunol., June 1, 2008; 180(11): 7558 - 7564. [Abstract] [Full Text] [PDF]

				I. Godinez, T. Haneda, M. Raffatellu, M. D. George, T. A. Paixao, H. G. Rolan, R. L. Santos, S. Dandekar, R. M. Tsolis, and A. J. Baumler T Cells Help To Amplify Inflammatory Responses Induced by Salmonella enterica Serotype Typhimurium in the Intestinal Mucosa Infect. Immun., May 1, 2008; 76(5): 2008 - 2017. [Abstract] [Full Text] [PDF]

				E. A. Miao, R. K. Ernst, M. Dors, D. P. Mao, and A. Aderem Pseudomonas aeruginosa activates caspase 1 through Ipaf PNAS, February 19, 2008; 105(7): 2562 - 2567. [Abstract] [Full Text] [PDF]