HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 47, 34104-34119, November 23, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/47/34104    most recent M702238200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, Y.-F. Articles by Herr, J. C. Search for Related Content PubMed PubMed Citation Articles by Li, Y.-F. Articles by Herr, J. C. Social Bookmarking                      What's this?

FSCB, a Novel Protein Kinase A-phosphorylated Calcium-binding Protein, Is a CABYR-binding Partner Involved in Late Steps of Fibrous Sheath Biogenesis^*

Yan-Feng Li¹, Wei He^¶1, Kula N. Jha, Ken Klotz, Young-Hwan Kim, Arabinda Mandal, Silvia Pulido, Laura Digilio, Charles J. Flickinger, and John C. Herr²

From the Center for Research in Contraceptive and Reproductive Health, Department of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, the Department of Urology, Daping Hospital, The Third Military Medical University, Chongqing 400042, China, and the ^¶Department of Obstetrics and Gynecology, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China

We report characterization of a novel testis- and sperm-specific protein, FSCB (fibrous sheath CABYR binding), that is expressed post-meiotically and localized in mouse sperm flagella. FSCB was identified as a binding partner of CABYR, a calcium-binding protein that is tyrosine-phosphorylated during capacitation. Orthologous genes of FSCB are present in other mammals, including rat and human, and conserved motifs in FSCB include PXXP, proline-rich and extensin-like regions. FSCB is phosphorylated by protein kinase A as shown by in vitro phosphorylation assay and also by determining phosphorylation sites in native FSCB from mouse sperm. Calcium overlay assay showed that FSCB is a calcium-binding protein from sperm. FSCB is a post meiotic protein first expressed at step 11 of mouse spermatogenesis in the elongating spermatids, and it subsequently incorporates into the flagellar principal piece of the sperm. Ultrastructurally, FSCB localized to a cortical layer of intermediate electron density at the surface of the ribs and longitudinal columns of the fibrous sheath. Due to its temporal appearance during spermiogenesis and location at the cortex of the fibrous sheath, FSCB is postulated to be involved in the later stages of fibrous sheath assembly.

Received for publication, March 14, 2007 , and in revised form, July 25, 2007.

^* This work was supported by National Institutes of Health (NIH) Grant U54 29099 and by Grant D43 TW/HD 000654 from the Fogarty International Center, NIH. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) EF133693 [GenBank] .

¹ Both authors contributed equally to this work.

² To whom correspondence should be addressed: Dept. of Cell Biology, University of Virginia, P. O. Box 800732, Charlottesville, VA 22908. Tel.: 434-924-2007; Fax: 434-982-3912; E-mail: jch7k{at}virginia.edu.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Kawashima, B. A.H. Osman, M. Takashima, A. Kikuchi, S. Kohchi, E. Satoh, M. Tamba, M. Matsuda, and N. Okamura CABS1 Is a Novel Calcium-Binding Protein Specifically Expressed in Elongate Spermatids of Mice Biol Reprod, June 1, 2009; 80(6): 1293 - 1304. [Abstract] [Full Text] [PDF]