HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 282, Issue 47, 34315-34324, November 23, 2007

This Article Full Text Full Text (PDF) All Versions of this Article: 282/47/34315    most recent M706486200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nakamata, K. Articles by Yoda, K. Search for Related Content PubMed PubMed Citation Articles by Nakamata, K. Articles by Yoda, K. Social Bookmarking                      What's this?

KEG1/YFR042w Encodes a Novel Kre6-binding Endoplasmic Reticulum Membrane Protein Responsible for -1,6-Glucan Synthesis in Saccharomyces cerevisiae^*

From the Department of Biotechnology, University of Tokyo, Yayoi, Bunkyo-Ku, Tokyo 113-8657, Japan

KEG1/YFR042w of Saccharomyces cerevisiae is an essential gene that encodes a 200-amino acid polypeptide with four predicted transmembrane domains. The green fluorescent protein- or Myc₆-tagged Keg1 protein showed the typical characteristics of an integral membrane protein and was found in the endoplasmic reticulum by fluorescence imaging. Immunoprecipitation from the Triton X-100-solubilized cell lysate revealed that Keg1 binds to Kre6, which has been known to participate in -1,6-glucan synthesis. To analyze the essential function of Keg1 in more detail, we constructed temperature-sensitive mutant alleles by error-prone polymerase chain reaction. The keg1-1 mutant cells showed a common phenotype with kre6 mutant including hypersensitivity to Calcofluor white, reduced sensitivity to the K1 killer toxin, and reduced content of -1,6-glucan in the cell wall. These results suggest that Keg1 and Kre6 have a cooperative role in -1,6-glucan synthesis in S. cerevisiae.

Received for publication, August 6, 2007 , and in revised form, September 17, 2007.

^* This work was supported by a grant-in-aid for Scientific Research from the Japan Society for the Promotion of Science (to Y. N.), grants for "Bioarchitect Research" from the Institute of Physical and Chemical Research (RIKEN) of Japan (to K. Y.), and a grant from the Noda Institute of Scientific Research (to K. Y.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed. Tel.: 81-3-5841-8138; Fax: 81-3-5841-8008; E-mail: asdfg{at}mail.ecc.u-tokyo.ac.jp.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. Kitamura, K. Someya, M. Hata, R. Nakajima, and M. Takemura Discovery of a Small-Molecule Inhibitor of {beta}-1,6-Glucan Synthesis Antimicrob. Agents Chemother., February 1, 2009; 53(2): 670 - 677. [Abstract] [Full Text] [PDF]

				M. Takeuchi, Y. Kimata, and K. Kohno Saccharomyces cerevisiae Rot1 Is an Essential Molecular Chaperone in the Endoplasmic Reticulum Mol. Biol. Cell, August 1, 2008; 19(8): 3514 - 3525. [Abstract] [Full Text] [PDF]