| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 282, Issue 5, 2956-2966, February 2, 2007
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12
From the
Graduate School of Life Science, Hokkaido University, Sapporo 001-0021 and the Department of Biochemistry, Kobe Pharmaceutical University, Kobe 658-8558, Japan
Accumulating evidence suggests the involvement of chondroitin sulfate (CS) and dermatan sulfate (DS) hybrid chains in the brain's development and critical roles for oversulfated disaccharides and IdoUA residues in the growth factor-binding and neuritogenic activities of these chains. In the pursuit of sources of CS/DS with unique structures, neuritogenic activity, and therapeutic potential, two novel CS/DS preparations were isolated from shark liver by anion exchange chromatography. The major (80%) low sulfated and minor (20%) highly sulfated fractions had an average molecular mass of 3.8–38.9 and 75.7 kDa, respectively. Digestion with various chondroitinases (CSases) revealed a large panel of disaccharides with either GlcUA or IdoUA scattered along the polysaccharide chains in both of the fractions. The higher Mr fraction, richer in IdoUA(2-O-sulfate)
1–3GalNAc(4-O-sulfate) and GlcUA
/IdoUA1–3GalNAc(4,6-O-disulfate) units, exerted greater neurite outgrowth-promoting (NOP) activity and better promoted the binding of various heparin-binding growth factors, including pleiotrophin (PTN), midkine, recombinant human heparin-binding epidermal growth factor-like growth factor, VEGF165, fibroblast growth factor-2, fibroblast growth factor-7, and hepatocyte growth factor (HGF). These activities were largely abolished by digestion with CSase ABC or B but only moderately affected by a mixture of CSases AC-I and AC-II. In addition, the NOP activity of the larger fraction was markedly reduced by desulfation with alkali, suggesting a role for the 2-O-sulfate of IdoUA(2-O-sulfate)1–3GalNAc(4-O-sulfate). The NOP activity of the higher molecular weight fraction and that of the embryonic pig brain-derived CS/DS fraction were also sup pressed to a large extent by antibodies against HGF, PTN, and their individual receptors cMet and anaplastic lymphoma kinase, revealing the involvement of the HGF and PTN signaling pathways in the activity.
Received for publication, October 2, 2006 , and in revised form, November 30, 2006.
* This work was supported in part by Grants-in-aid 17659020, 16390026, and 14082207 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, the Core Research for Evolutional Science and Technology program of the Japan Science and Technology Agency, and the Human Frontier Science Program. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
1 Supported in part by a postdoctoral fellowship from the Japan Society for the Promotion of Science.
2 To whom correspondence should be addressed: Laboratory of Proteoglycan Signaling and Therapeutics, Graduate School of Life Science, Hokkaido University, Frontier Research Center for Post-Genomic Science and Technology, Nishi 11-choume, Kita 21-jo, Kita-ku, Sapporo, Hokkaido 001-0021, Japan. Tel.: 81-11-706-9054; Fax: 81-11-706-9056; E-mail: k-sugar{at}sci.hokudai.ac.jp.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Tone, L. C. Pedersen, T. Yamamoto, T. Izumikawa, H. Kitagawa, J. Nishihara, J.-i. Tamura, M. Negishi, and K. Sugahara 2-O-Phosphorylation of Xylose and 6-O-Sulfation of Galactose in the Protein Linkage Region of Glycosaminoglycans Influence the Glucuronyltransferase-I Activity Involved in the Linkage Region Synthesis J. Biol. Chem., June 13, 2008; 283(24): 16801 - 16807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Catlow, J. A. Deakin, Z. Wei, M. Delehedde, D. G. Fernig, E. Gherardi, J. T. Gallagher, M. S. G. Pavao, and M. Lyon Interactions of Hepatocyte Growth Factor/Scatter Factor with Various Glycosaminoglycans Reveal an Important Interplay between the Presence of Iduronate and Sulfate Density J. Biol. Chem., February 29, 2008; 283(9): 5235 - 5248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Minamizaki, T. Mizoguchi, T. Goto, H. Tamiaki, and Y. Fujita Identification of Two Homologous Genes, chlAI and chlAII, That Are Differentially Involved in Isocyclic Ring Formation of Chlorophyll a in the Cyanobacterium Synechocystis sp. PCC 6803 J. Biol. Chem., February 1, 2008; 283(5): 2684 - 2692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Fongmoon, A. K. Shetty, Basappa, S. Yamada, M. Sugiura, P. Kongtawelert, and K. Sugahara Chondroitinase-mediated Degradation of Rare 3-O-Sulfated Glucuronic Acid in Functional Oversulfated Chondroitin Sulfate K and E J. Biol. Chem., December 21, 2007; 282(51): 36895 - 36904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yamada, H. Morimoto, T. Fujisawa, and K. Sugahara Glycosaminoglycans in Hydra magnipapillata (Hydrozoa, Cnidaria): demonstration of chondroitin in the developing nematocyst, the sting organelle, and structural characterization of glycosaminoglycans Glycobiology, August 1, 2007; 17(8): 886 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S Deepa, S. Yamada, S. Fukui, and K. Sugahara Structural determination of novel sulfated octasaccharides isolated from chondroitin sulfate of shark cartilage and their application for characterizing monoclonal antibody epitopes Glycobiology, June 1, 2007; 17(6): 631 - 645. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
