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J. Biol. Chem., Vol. 282, Issue 52, 37308-37315, December 28, 2007

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A Poised Initiation Complex Is Activated by SNF1^*

From the Department of Biochemistry, University of Washington, Seattle, Washington 98195

Snf1, the yeast AMP kinase homolog, is essential for derepression of glucose-repressed genes that are activated by Adr1. Although required for Adr1 DNA binding, the precise role of Snf1 is unknown. Deletion of histone deacetylase genes allowed constitutive promoter binding of Adr1 and Cat8, another activator of glucose-repressed genes. In repressed conditions, at the Adr1-and Cat8-dependent ADH2 promoter, partial chromatin remodeling had occurred, and the activators recruited a partial preinitiation complex that included RNA polymerase II. Transcription did not occur, however, unless Snf1 was activated, suggesting a Snf1-dependent event that occurs after RNA polymerase II recruitment. Glucose regulation persisted because shifting to low glucose increased expression. Glucose repression could be completely relieved by combining the three elements of 1) chromatin perturbation by mutation of histone deacetylases, 2) activation of Snf1, and 3) the addition of an Adr1 mutant that by itself confers only weak constitutive activity.

Received for publication, September 4, 2007 , and in revised form, October 4, 2007.

^* This work was supported by NIGMS Grants 26079 and NIDDK67276 (to E. T. Y.) and by NIGMS Grant PHS NRSA T32 GM07270 (to R. K. B.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement"in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains two supplemental figures and two tables.

This article was selected as a Paper of the Week.

¹ To whom correspondence should be addressed: Dept of Biochemistry, Box 357350, University of Washington, Seattle, WA 98195-7350; Fax: 206-685-1792; E-mail: ety{at}u.washington.edu.

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				R. K. Biddick, G. L. Law, K. K. B. Chin, and E. T. Young The Transcriptional Coactivators SAGA, SWI/SNF, and Mediator Make Distinct Contributions to Activation of Glucose-repressed Genes J. Biol. Chem., November 28, 2008; 283(48): 33101 - 33109. [Abstract] [Full Text] [PDF]

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