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J. Biol. Chem., Vol. 282, Issue 52, 37605-37617, December 28, 2007

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Identification and Characterization of a Juvenile Hormone Response Element and Its Binding Proteins^*

Yiping Li, Zhaolin Zhang, Gene E. Robinson, and Subba R. Palli¹

From the Department of Entomology, University of Kentucky, Lexington, Kentucky 40546 and the Department of Entomology and Neuroscience Program, University of Illinois, Urbana, Illinois 61801

Juvenile hormones (JH) regulate a wide variety of developmental and physiological processes in insects. Comparison of microarray data on JH-induced genes in the fruit fly, Drosophila melanogaster, L57 cells and in the honey bee, Apis mellifera, identified 16 genes that are induced in both species. Analysis of promoter regions of these 16 D. melanogaster genes identified DmJHRE1 (D. melanogaster JH response element 1). In L57 cells, the reporter gene regulated by DmJHRE1 was induced by JH III. Two proteins (FKBP39 and Chd64) that bind to DmJHRE1 were identified. FKBP39 and Chd64 double-stranded RNA inhibited JH III induction of a reporter gene regulated by DmJHRE1. FKBP39 and Chd64 proteins expressed in yeast bound to DmJHRE1. Two-hybrid and pull-down assays showed that these two proteins interact with each other as well as with ecdysone receptor, ultraspiracle, and methoprene-tolerant protein. Developmental expression profiles and JH induction of mRNA for FKBP39 and Chd64 proteins and their interaction with proteins known to be involved in both JH (methoprene-tolerant protein) and ecdysteroid action (ecdysone receptor and ultraspiracle) suggest that these proteins probably play important roles in cross-talk between JH and ecdysteroids.

Received for publication, June 5, 2007 , and in revised form, October 22, 2007.

^* This project was supported by National Institutes of Health Grant RO1 GM070559-02 (to S. R. P.). This is contribution 07-08-125 from the Kentucky Agricultural Experimental Station. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

¹ To whom correspondence should be addressed: Dept. of Entomology, University of Kentucky, Lexington, KY 40546-0094. Fax: 859-323-1120; E-mail: rpalli{at}uky.edu.

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